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We narrowed to 1,010 results for: RING;

Showing: 211 - 220 of 1010 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...vector into the culture medium during adeno-associated virus manufacturing. Human Gene Therapy, 21(10), ... iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their...check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and purified ...titer of your viral vector solution. A variety of titering methods have been developed to determine the titers...TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...outcome and planning.  Both ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration...has to be digested with DNase before undergoing titering, to remove residual plasmid DNA carried over from...

  2. PhD Applications After COVID

    Type
    Blog Post
    ...worry about traveling during an already stressful time, and I was more relaxed during both sets of interviews...yourself to a few members of your desired department during the application process. Many institutions recommend...in case one isn’t able to respond to your email. During my PhD application cycle, I reached out to around... and make sure you cover everything you need to during the interview. (Virtual) Campus Visits After receiving... financial and logistical obstacles could make touring potential programs and universities stressful or...Through the virtual systems created and promoted during COVID-19, online meetings with current students...worked with students to maintain these time goals during COVID-19? How are students funded? Are there any...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...genome engineering across multiple species Recent discoveries in the fields of genome engineering and synthetic...multiplex automated genome engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate...gene of interest. Although the parts involved in altering gene expression are relatively well known, particularly...labs who wish to harness this powerful genome engineering tool. Bassett, et al. J Genet Genomics. 2015...loci (multiplexing). Currently, the only genome engineering method in bacteria that enables rapid, automated...combinatorial genomic libraries. MAGE has allowed genome-engineering endeavours of unparalleled complexity in Escherichia...mutant enables transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... with limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I...textbooks” (Brian, 1996). But is it possible to bring the kinds of transformative educational experiences...dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable, easily purified protein...interest to other researchers in their field. Indeed, sharing results with the broader scientific community represents...all shaped his teaching style, with the former offering excellent training in improvisation and the latter...Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects MDH-focused CUREs at...available technical and pedagogical resources, offers mentoring for instructors, and provides opportunities for...

  5. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...community. Sharing is caring We’re thrilled that our depositors reap the benefits of sharing plasmids with...hooked on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's...plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly special...honor the creativity, passion, and dedication to sharing epitomized by the creators of these plasmids with...appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who...deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming chore...Flame Award winners- we’d like to thank you for sharing your plasmids at Addgene. You are the reason we...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...mix. During the incubation the immobilized antibody binds to the target protein, thereby tethering it to...approaches allow for optimized strategies for differing amounts of target proteins or antibody bonding...immunoprecipitation and simply refers to the process of anchoring an antibody, often called the capture antibody...eliminating the risk of the primary antibody interfering with downstream assays. This is particularly ...for an IP, samples are typically lysed in a non-denaturing buffer containing non-ionic detergents, such ...proteins, including those in the nucleus, more stringent buffers may be needed. Since many factors, including...Frequently, the antibodies used for a standard, denaturing Western blot will not be suitable for an IP, ...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...team building and bring back some degrees of belonging that was probably lost during these weird times...Discussing data with colleagues can be more difficult during the pandemic era, with fewer opportunities for ...   Implement new tools for creative interaction During Zoom meetings, different tools can be used to promote...The annotate function can also make the screen-sharing experience more interactive. The button is on the...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes.... This document can be shared and serve as a springboard for future discussion. Collaboration and creativity...great functionality that is widely used and could bring back a taste of that casual kitchen chatter, ultimately...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...immune system to fight progressing cancers. It was inspiring to participate in the 2017 Cell Symposium on Cancer... are coming together to tackle the challenge of curing cancer in cross-disciplinary projects and collaborations...reflect how cancer/immunology researchers are preparing and carrying out this multi-pronged attack. Inflammation...our recent post “Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra...CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor...Professor Michel Sadelain from the Memorial Sloan Kettering Cancer Center. In contrast to using randomly integrating..., CRISPR/Cas9 genome editing allows targeted engineering of the TCR locus. He also highlighted the UCSF...

  9. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...to all of our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs ...labs currently sharing viral preps) through the Addgene repository. Without these scientists and the support...to have such an impact on scientific materials sharing. I would also like to recognize a few scientists...Addgene has been successful in helping thousands of sharing scientists from laboratories all over the world...Scientist Team has been developing expertise in answering questions on virus and helping provide educational...reach scientists around the world. Our Software Engineering Team (and, added in 2018, our Product Team) continues...Maintaining our excellent service and accuracy involves caring and careful Addgenies, but also a lot of good tracking...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...the work by using the principles of recombineering. In recombineering, bacteria express phage recombination...REPLACR-mutagenesis, harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions...how to replace your SDM method with REPLACR (Recombineering of Ends of Linearized Plasmids After PCR). ...modification by homologous recombination. In most recombineering strategies, only 3-4 phage proteins are needed...directly transformed into bacteria expressing recombineering proteins Red/ET from a temperature-sensitive...bacteria are subsequently grown at 37 °C, the recombineering plasmid is not maintained. Recombination efficiency... the need for selection usually required in recombineering. REPLACR-mutagenesis is also well suited for...
Showing: 211 - 220 of 1010 results