We narrowed to 470 results for: Mag;
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TypeBlog Post...encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020. Broad tropism: ...
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Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post... Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience... -
Plasmids 101: Codon usage bias
TypeBlog Post...transcription factor binding sites) As you might imagine, it’s not easy for humans to balance all of these... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell... -
The Challenges of Cell Culture
TypeBlog Post... all the major cell lines on public databases. Image attributions (from top to bottom) Database Center... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...receives stimulation in one of the two chambers (see image below). If a circuit is reinforcing, the animal ... -
Plasmids 101: Control Plasmids
TypeBlog Post...the protein. For example, fluorescence microscopy images resulting from our experiment that includes the... -
Fluorescence Titering Assay
TypeProtocol...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...control option. Total protein stains or stain-free imaging systems are both effective for measuring the total... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS. But MACS... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post... the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...keeping, ELNs offer functionalities that are hard to imagine on paper, such as instant search through all saved... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post... recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered... -
Transferable Skills Guide: Time Management
TypeBlog Post...university. My to-do list might look something like the image on the right (and for those of you who can’t read... -
Five Popular Model Organisms
TypeBlog Post...Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis... -
Plasmids 101: Biotinylation
TypeBlog Post...PMCID: PMC307283. 13. Howarth, M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, ... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...introduce all desired codon changes (step 1 in the image above). A second universal primer is used to synthesize... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...interactions between these proteins is hard to imagine yet alone study. To elucidate these interactions...