We narrowed to 949 results for: app

...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements...

...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

...on OCRL1 already underway in his lab. The gene appears to play a role in many cell processes involving...In Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic...

...would apply a percentage of returns to support student projects, but it quickly became apparent that raising...

...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...

...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day...

...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ...

...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer...

...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways...

...referred to objects that exist online, but this approach has been adapted for the identification of physical... Addgene plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats...

...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people...

...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ...

...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...

...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...

...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this...

...Juan R. Torruella, U.S. First Circuit Court of Appeals. She served as in-house counsel at St. Elizabeth...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ...

...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different...

...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first...

...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves...

...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...

...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given...