Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 970 results for: Abo;

Showing: 421 - 440 of 970 results

  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series...both changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased...change C to T, G to A, A to G, and T to C, represent about two-thirds of known pathogenic point mutations. ...

  4. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the 2018 Chromosome...lot of scientists who not only remember to think about DNA in this context, but also specifically study...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 ...chromatin This is not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...this paper are available at Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources... Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find...

  6. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you tell me where to start... for scientific exploration, discovery, and collaboration. One module currently under development comes...circuits and logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL)... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ... called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant...troubleshooting methodologies drew me to learn more about the process that was used to create this important...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

  9. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...Addgene blog Learn about using nanobodies to control protein activity Read about RANbodies, reporter ...reporter nanobody fusions Learn more about using the secondary nanobody toolbox for immunodetection Resources...Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics guide ...

  10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...the name, date, and location of all analyses (see above). Give every experiment its own directory: In ...record your goal, approach, and conclusions (see above). Keep original files in a separate folder: You... can simply make a note in your methods section about the edit that was temporarily used. Just be sure... Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time and Track Your...

  11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

  12. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

  13. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

  14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...transitioned away from the bench and became all about finding the best product-market fit for this set...Notably, the team must have an upfront conversation about its goals and vision for the company; this will ...section! And read on for more great information about starting a biotech company. Suggested reading The...of Sample6) for taking the time to speak with us about the biotech startup process and work environment...

  15. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragment are we talking about here? Many of the Fab fragments described above can be used for similar ...fragments are produced by proteolytic digestion above the hinge-region, which results in two independent...you consider which tools to use, be sure to think about what pieces of the antibody you do and don’t need...

  16. The time and cost required to make a plasmid

    Type
    Blog Post
    ...cloning before you can start your experiment? What about all the reagents you need to order? Sometimes, it...dive deep into generating the data you’re excited about. When we were closing in on the one million plasmids...million plasmids shared than not!), we think it’s about time we updated this post. How much time does ...choose the right cloning method Find blog posts about scientific sharing here Download the Plasmids 101...

  18. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...source of monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody... particular applications. Read on to learn more about each reporter or check out table 1 for a summary...Additional Resources on the Addgene Blog Learn more about using the secondary nanobody toolbox for immunodetection...can be found here Watch this video to learn more about nanobodies Additional Resources Looking for primary...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...August 2nd in Nature gives us even more to talk about, as it represents another step towards CRISPR germline...impossible with current tools, thus quieting some fears about the creation of “designer babies.” The differences...of individuals with varied backgrounds. Talking about this study with non-biologist friends Like me, you... Blog Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes...

  20. Summer SciComm: Preprints

    Type
    Blog Post
    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...key routes for researchers to share information about the emerging COVID-19 pandemic quickly and openly...awareness of the benefits of preprints. Some worry about faulty work being posted as preprints, assuming ...submission. Preprints at Addgene Preprints are all about sharing scientific knowledge faster and more openly...
Showing: 421 - 440 of 970 results