...TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based expression vector containing N-terminal, TEV cleavable...Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega. Contain a variety ...

...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...

...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...

...nucleotide level but share an overall structure of 13 base pair palindromic repeats separated by an 8 bp asymmetric...2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed...

...get its name? Often times, a gene gets its name based on what happens to an organism when a gene/protein.... Scientists reconstructed a transposase system based on the naturally mutated and non-functional transposase...

...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...

...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...

...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...

...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...

...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...

...team creates stabs for each plasmid being shipped based on the information in our lab software, labeling...

...- 5' = 28 bp We also need to include additional bases to complement the overhangs generated when digesting...

...Scientific Officer “Choose your PhD lab carefully. Don’t base it solely on the project. Assess whether or not ...

..., and now researchers have reported a mechanism based on studies of transgenic mice that helps to explain...

...organism. We have designed a Golden Gate system based on the daily needs of an advanced molecular plant...

...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...

... viral genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create...

...cells are classified as pluripotent or multipotent, based on their ability to generate different cell lineages...Cell research. You can search or sort this table based on plasmid name, species, gene name, and more. Plasmid...

...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1...

...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...