We narrowed to 571 results for: BLI
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  TypeBlog Post... selected and ranked based on the previously established sgRNA rules and potential on- and off-target...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier... - 
  
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug... - 
  
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,... - 
  
Summer SciComm Series: Modes of Communication
TypeBlog Post...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ... - 
  
Getting to Know Expansion Microscopy
TypeBlog Post...experiment or evaluating ExM images in a new publication. Why Expansion Microscopy? Traditional... - 
  
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ... - 
  
DNA Purification Without a Kit
TypeBlog Post...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA... - 
  
MXS Chaining
TypeBlog Post...high-throughput cloning strategies available for assembling these modular types of constructs, and different... - 
  
Lentiviral Vector Uses and Overview
TypeBlog Post...McGarrity GJ, Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl... - 
  
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ... - 
  
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes.... - 
  
CRISPR Plasmids - Drosophila
TypeCollection...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression... - 
  
CRISPR Plasmids - Prime Edit
TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable... - 
  
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-... - 
  
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...expression of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive... - 
  
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ... - 
  
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b... - 
  
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...have been used successfully in experiments and published in peer reviewed journals. If you need to generate... - 
  
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...