We narrowed to 569 results for: BLI;

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier...

...PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug...

...high-throughput cloning strategies available for assembling these modular types of constructs, and different...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ...

...experiment or evaluating ExM images in a new publication.         Why Expansion Microscopy?  Traditional...

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

...McGarrity GJ, Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl...

...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...

...expression of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive...

...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...

...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...