Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 644 results for: CAR

Clear keywords
Showing: 421 - 440 of 644 results

  4. Editor's Choice, October 2016

    Type
    Blog Post
    ...Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of Life Sciences...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...attached to the dish surface and should be handled carefully. Avoid touching the cells when replacing media...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE...incubator and pour the media into a waste container. Carefully add the OptiMEM + DNA + PEI solution to the CS5...cells (not poured on them) and can be adjusted by carefully tilting the CS5 back and forth. Pro-Tip To help...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Bio-Rad, 1863005 DG8 cartridge, Bio-Rad, 1864008 DG8 gasket, Bio-Rad, 1863009 DG8 cartridge holder, Bio-Rad... Generating the Droplets Place a DG8 cartridge into the cartridge holder. Using a 2–50 µL multichannel...Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to... centrifuge and place on ice. Wipe down a DG8 cartridge holder with bleach and place it in the Biological...Add 16 µL of the master mix to each PCR tube. Be careful to dispense to the bottom of the tube without collecting...reaction mixtures into the middle wells of the cartridge. Add 800 µL of droplet generation oil to a polystyrene...
Showing: 421 - 440 of 644 results