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We narrowed to 771 results for: Dos;

Showing: 421 - 440 of 771 results

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...Choosing a topic and gauging interest The first thing to do if you’ve thought about starting a journal club is...make. How often will you hold it? How many people do you expect to attend? What is the expectation on ...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...use. This will prevent spills, but, if any spills do occur, they should be wiped with 70% ethanol immediately...morphologies, being familiar with these morphologies and doing a quick check under the light microscope will help...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...they are present at a very low concentration. So how do we make sure to catch all of these potential mishaps...expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression ...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   ..., you will not be able to use far red-FPs. If you do not have a filter that will pass blue light to the...

  10. Cloning

    Type
    Guide
    ...plasmids for replication and protein expression. So how do scientists recombine DNA? There are many methods ...so dissimilar from restriction enzyme cloning). To do this your DNA fragment must first be amplified with...move it into a series of plasmids that allow you to do many different molecular biology techniques (such...

  11. CRISPR Plasmids - Tagging

    Type
    Collection
    ... homology arms and EGFP are available at Addgene. Do you have suggestions for other plasmids that should...

  12. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...life. That’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would...to express your protein of interest. However, you do still need a species-compatible promoter for expressing...

  13. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...five of these Cas proteins, while type I-C Cascades do not contain Cas6. Regardless of the subtype, the ...to the multi-Cas mechanism. These benefits of Cas3 do come at a cost — the previously mentioned issues ...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular biologists working with ...in order to understand what these vector particles do once they come in contact with target cells (Fig....

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

  17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluoresce, the Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro...recognition of ions or particularly small molecules that do not generate an immune response. Aptamers can be ...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...
Showing: 421 - 440 of 771 results