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We narrowed to 830 results for: EED

Showing: 421 - 440 of 830 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...confirmed that avidin was indeed the culprit in egg white injury by feeding rats purified avidin (Kresege...external sources. Humans absorb most of the biotin they need in the small intestine from the microflora that ...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...backbone: the DNA vector that contains everything needed to replicate inside a host cell but with space ....  We built these plasmids for flexibility and speed, so you can design, test, and innovate faster than... Perhaps these antibodies are just the tools you need to push the field forward! Browse the IPI Glypican...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...plasmid compared to DH5α. However, more research is needed to determine which recA- strains are most effective...2009), pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple...production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church lab uses this system...Response Element (TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is...linked in groups of 6), allowing transcription to proceed. When cumate is added, it binds cTA, resulting ...

  5. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA sequence encodes the proteins the transposon needs for transposition. Transposition protein: This is... transposons” since they encode the protein they need to move around, either a transposase or a reverse...transcriptase. Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...end-joining activity, and for others, there is a need for the exclusive ligation of fully base-paired ...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    ...packaging capacity, AAV vectors were suitable for our needs for the following reasons: Safety: Since AAVs ...should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are relatively quick...useful for a wide variety of experiments, care still needs to be taken when selecting which particular AAVs...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...as excitation wavelengths and emission profiles, need to be matched with the experimental setup and objectives...Resonance Energy Transfer (FRET) applications, the dyes need to have compatible excitation and emission profiles...witnessed remarkable advancements, driven by the need for more precise, stable, and versatile imaging ...

  9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more... injury in the acutely inflamed glomerulus." Proceedings of the National Academy of Sciences (2016): 201606253...cell subsets in the skin-draining lymph node." Proceedings of the National Academy of Sciences 113.4 (2016...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the end, this results in an experiment that could need more than 8x107 successfully targeted cells. Depending...the analysis becomes easier, the number of cells needed is lower, and perhaps most important, targeting...good idea to keep a bioinformatician close if you need help putting your list together. Once you have your...

  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...should also be considered. If the light intensity needed for activation is too high, the photoactivation...photoswitching between two fluorescence wavelengths. Indeed, within the world of super resolution microscopy...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

  12. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...grow haywire, access and create more vasculature to feed themselves, use the blood stream to commute to and...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted... inhibit expression of co-stimulatory molecules needed for T cell effector function. Cancer cells also...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...we’ve included a glossary at the end if you ever need to double-check your vocabulary! A brief review ...double-strand breaks. The only things base editors really need from Cas9 are sequence specificity and the R-loop...efficient and precise editors. Most researchers agreed on the first part of the likely editing mechanism...

  15. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...should our new blog be any different? If you still need an extra push, there’s evidence that laughter really...’s a way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that ...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was...how seriously we take costume design. In case you need more convincing, I’ve got just the proof: Above...

  18. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...choosing the cargo, serotype, and prep size they need for their experiments — and we’ve been able to ship...continue to expand the collection to meet the diverse needs of our community and support a wide range of experimental...

  19. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...in interactive figures is a great step up, but I needed a way to show different aspects of the data simultaneously... R can further customize the dashboard to their needs. I hope you find the dashboard useful when analyzing...

  20. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...biologists alike. Now with over 600 articles, we needed a better way to organize and present the vast collection...outside of these five areas and you can find what you need by field (ex: microbiology, neuroscience), or explore...
Showing: 421 - 440 of 830 results