We narrowed to 638 results for: Erf

1. Sleeping Beauty Awakens for Genome Engineering

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   ... is one such system which has been designed to perform gene transfer in vertebrates. The system is comprised...

2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ... history of code making and code breaking is wonderfully analogous to genetic circuits discussed at this...

3. Management for Scientists: What Makes a Good Manager Anyway?

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   ... Sometimes this means you have to fire a good performer if they are enough of a jerk to make the rest ...

4. Advice for Choosing a Research Project

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   ...pro in a field, but to be trained to think and perform like a scientist. Researchers should  pick a topic...

5. The Stingy Scientist: How the Baby Gel Box Was Born

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   ...country and we are quick to throw out chipped or imperfect equipment.  We can’t safely recycle these materials...

6. Plasmids 101: Antibiotic Resistance Genes

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   ...Reference Pages View our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

7. Antibodies 101: Epitope Tags

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   ...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

8. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is even able to ...

9. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   ...IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie...

10. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does have some...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    ... a strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter...

12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ... gRNA sequences. This allowed tracing of the performance of each gRNA multiple times within the same experiment...

13. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ...sequencing to determine serotype AAV packaging is not perfect and while we think of the AAV vector genomes as...

14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    ...injection. How long post-injection did you wait until performing the experiment? What detection methods did you...

15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...elements Read about four considerations for the perfect AAV tool Learn about synthetic promoter AAVs for...

16. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

17. Summer SciComm: Science Storytelling

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    ... drives a story forward - and this drive is so powerful that it can entice an audience to come along on...

18. Addgene Begins Distribution of Recombinant Antibodies

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    ... about the antibody, the target antigen, and performance in various applications are included in the full...

19. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...viral service but did you know that Addgene also performs independent research? Since launching the viral...

20. Using Addgene's New Viral Service for Your Research

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    ... gene, replace it, modulate its expression, or perform a genome-wide screen, we’ve got viruses to help...