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We narrowed to 520 results for: HA tag

Showing: 421 - 440 of 520 results

  1. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...fluorophores to visualize multiple targets at once. An advantage of multiplexing is that you can ask how multiple...choose can make or break your experiment, so take advantage of all the available resources and give yourself...

  2. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and MANCOVA...

  3. CRISPR Library Amplification

    Type
    Protocol
    ... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2...Caution Electroporation involves the use of high voltages, please use caution when activating pulse and ...down at 30 ℃ overnight. Critical Ensure at this stage that no unabsorbed media drips onto the lid. Let...

  4. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...would not end up in a life sciences career but life has a way of making unexpected decisions. As someone ... I was able to express my visions for what my Instagram posts would look like. While working remotely ...continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role... Some of recent Meet an Addgenie posts on Instagram! From this project, I observed what it was ...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...Retroviridae family. Infection with lentiviruses has a long incubation period that eventually leads to... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...their stable expression and wide tropism. Addgene has an extensive collection of lentiviral CRISPR libraries...information (including tropism and viral genome percentage) and resources on viral safety. Resources and...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR..., both techniques offer researchers different advantages and limitations.  The four main factors in which...allow for light delivery. Chemogenetics poses the advantage of being less invasive and more flexible as it...controlled EndogenousNot easily controlled Unique advantages Mapping the brainDissecting circuits Studying...

  7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-1 to deplete...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-... treatment with CRISPR/Cas9 SAM increased the percentage of cells with activated HIV-1, measured via HIV...

  8. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...characterization of over 1,000 mutations, and the group has deposited several hundred plasmids from this study...lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (... any mutations that that confer proliferation advantages will be revealed in the absence of growth factor...

  9. Our New Antibody Mascot is....

    Type
    Blog Post
    ...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...submit your name suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...monosynaptically from these cells. These modifications take advantage of the fact that RABV is an enveloped virus. In...

  11. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Request  has seen a fantastic response from the research community. Customers have taken advantage of the...

  13. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...scFv. Each time the reporter is translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP..., as the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the Brightest...

  14. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ... AAV quality control Historically, AAV vector QC has been sparse including only titration of AAV particles... used SMRT sequencing, TA-based ligation and tagmentation-based methods for AAV genome sequencing (Tai...self-complementary AAVs and the TA-based ligation and tagmentation preparation approaches described previously ...single-stranded and both TA-based ligation and tagmentation require a double-stranded template.  Using viral...the mix to serve as effective substrates for tagmentation-based library preparation procedures. This finding...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    ...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...retreat to the woods for months at a time, so take advantage of whatever time you have. If you’re like me, ...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...normally found at low abundance in E. coli. The advantage of producing additional tRNAs is that you can ...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...properties of the individual Cas9. They also mutagenized the cytidine deaminase portion of the base editor...

  19. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership ...just have to know it when you see it? “The Three Stages of Your Career: Doer, Manager, Leader” by Charlotte...importance of these very different roles. At any stage in our careers we may be in a cycle of doing, managing...

  20. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...in state machine technology? Biological research has produced a massive amount of information regarding...two-input gene regulation programs. The software has two components - a database of pre-compiled register...reporter-based) each come with their advantages and disadvantages. Sanger sequencing is simple and the...
Showing: 421 - 440 of 520 results