We narrowed to 988 results for: IGH@

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... TX-TL system is capable of robust expression of high G+C content genes, as validated through monitoring...CRISPR Plasmids and Resources page. Here are some highlights from the past couple months including several...

...are very valuable biological tools, and with the right safety considerations, they aren't dangerous for...transductions can offer benefits such as tissue-specificity, high efficiency, and can be selected to be either integrating...our viral selections to see if we have one that might work for your next experiment. Using transduction...Answering these questions will help guide you to the right method for your experiment.  A cool feature of viral...virus can sound daunting, but it is easier than you might think. We have protocols available to get you started...

...Who doesn’t love a library? A traditional library might contain thousands or millions of different books...region. This makes them useful for a wide variety of high-throughput experiments. There are currently 390 ...libraries can be designed for all kinds of different high-throughput experiments. Our most popular screening...out our blog post on this library. Let’s also highlight a prokaryotic library: the Cultivarium MACKEREL...

...yet familiar. The idea that my piecemeal degree might one day foster a meaningful day job only came into...enjoyed a taste of what was to offer, continued straight on to a master's degree in science communication... one day required of me for work, I would have a high level of career satisfaction indeed.   For many ...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...law to poetry. A research discipline in its own right, science communication reflects a necessary confluence...

... be ideal for you. Here, we will chat about who might want to explore this role, how to seek out such ...commitment, planning, and funding – they may not be right or necessary for many scientists! Unless some of...from third parties or your home institute. Discuss rights Do you expect to generate data that will contribute...It is never too early to discuss authorship and rights for anything that could be generated during the...opportunity, but also a really good time! If it sounds right for you, start thinking outside of your current ...

...buck - it grows at higher cell densities than most of its counterparts, yielding higher production power... phaffii in the 1970s with the goal of producing high protein animal feed. Due to increasing prices of...flask volume. What’s even better? K. phaffii is highly efficient at protein secretion, making harvesting...knock-in or knock-out) is essential to your work, you might want to pick a yeast strain best suited to it. Putting...

...optimal for experiments requiring high signal to noise ratios, imaging of high frequency glutamate signaling...to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces stronger...properties The improved variants of iGluSnFR3 have higher expression levels and produce stronger fluorescent...Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit the Fluorescent Protein...

...with a large range of characteristics (color, brightness, photostability, maturation, oligomerization)...choosing a FP, but much of the primary data one might require when making such a decision remains spread...there is a great deal more information that one might like to store about FPs than is currently represented...multi-parametric search queries.  Looking for a bright, monomeric FP published in the last few years with... for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein Choosing Your Fluorescent...

...these visualization tools is CRISPR-Sirius, the brightest CRISPR-based tool to date for visualizing genomic...chromosomes in living cells, the system requires high-copy chromosome-specific loci in the genome and ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio...its predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic...copy genes. If you want to visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius ...

...grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all the optional...technical jargon that even scientists in neighbouring disciplines might struggle to understand. Why should ...whispered questions from friends and classmates back in high school, and it’s oddly fitting that I’ve ended up...

...figure to the right), we identified R-body mutants that extend at both lower and higher pH. We initially...putting the operon under a strong promoter results in high R-body expression  (Schrallhammer et al 2012). This...environmental conditions. More speculatively, R bodies might endow divergent organisms with new functions. For...understanding of how R bodies assemble and function, we might be able to endow cells with new ways to interact...

...sides of the break, we determined that Cas9 held tightly to three of the four strands of the cleaved duplex... the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing than single...have shown that this technique supports extremely high frequencies of genome cutting and, when donor DNA...included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol for nucleofection...Central PMCID: PMC4383097. 3. Kim, Sojung, et al. "Highly efficient RNA-guided genome editing in human cells...

...In a big lab, the advisor might not be around much and your real boss might be a postdoc – this isn't ...competitive style to spur you to succeed or you might enjoy working closely with others to accomplish ...before you take the position. Am I choosing the right mentor? Make sure your advisors will be good role...candidate to ask lab heads, lab members and lab neighbors during the interview process. What if I want...

... you to select cells with high GFP expression. These cells have the highest levels of Cas9 and gRNA expression... which method you use, the procedure will vary slightly.   Two Golden Gate options that are available...expression, and thus the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9.... “Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.”  Nature Biotechnology...al. “A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and...

.... If submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button..., and plasmid description/experimental purpose (right). On the article page, enter the name, type, and...The more sequence data available, the better. We highly encourage you to upload full sequence data whenever...cannot be propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone to recombination...Figure 12: The "Copy" button is located near the top right of each data entry page. Once you have completed...necessary by my institution or other third party rights holders. Once this process is complete, you will...

...Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology... cells, has become an established alternative to high-priced commercial devices.     Fig. 2: Workflow...Workflow for open software (left) vs. open hardware (right).    Taking this all together, the essential...open hardware. Upper left panel shows the path of light through a microscope. Lower left panel shows an ...differentiating monocyte with planes and hardware to it. Right panel shows workflow of open hardware.    Conclusion...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...close proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The...the AAV capsids (left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV.CAP-B capsids are...mammalian cells. Long sequences traditionally have higher risks of off-target mutations. These plasmids contain...

...to induce high levels of gene activation for both reporter and endogenous genes. It is highly specific ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...target only requires a small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the...

...hybridoma, you can choose one that produces high-affinity/high-avidity antibodies. The same hybridoma is...drive can be identified by sequencing the heavy and light chains. To circumvent any possible genetic drift...  Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity...validating monoclonal antibodies, their cost is higher than that of polyclonal antibodies. For example...

...realized that working in the wet lab wasn’t the right path for me. I began exploring alternative career...track and feel supported. Another team member was highly self-motivated and excelled when given the freedom... made similar transitions can provide you with insights, lessons from their experiences, and practical...perspectives and facilitated a team meeting to highlight their complementary strengths. By encouraging ...