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We narrowed to 1,038 results for: LAS

Showing: 421 - 440 of 1038 results

  1. 5 Science Rap Videos & Their Creator: Tom McFadden

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    Blog Post
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

  2. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...

  3. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... Surface labeling is particularly useful for classifying and sorting cells by identifying lineage markers...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live cells... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality for...infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk for other chronic...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

  5. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ...problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine base editors (CBEs...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

  6. Bringing Sustainable Practices to the Lab: Easy Action Items

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    Blog Post
    ...other scientists save resources by sharing plasmids Find plasmids at Addgene ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in ourselves... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

  7. 2022 and 2023: Reflections and Looking Forward

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    Blog Post
    ...and beyond. Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors...biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our fast-...knowledge and research opportunities. I have spent the last 9 months quietly learning about Addgene’s past and...

  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...AAV receptor (not strictly required)2 Human fibroblast growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1...pseudotypes are described with two numbers separated by a slash: the first number refers to the genome, and the ..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  9. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

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    Blog Post
    ...for at least a couple of decades. Green is the classic color, but fluorescent proteins are available in...in 2011 and then in another Nature Methods paper last year. The key to their bacterial phytochome-derived... could see deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable...the researchers described in Nature Communications last year, “The capability to control spectral properties...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team reported...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...

  12. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...associated publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor...11,000-fold. FRET biosensors are another common class of biosensors. Blue flame “cameleon” calcium sensors...compartment such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes...

  13. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post
    ...CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections...-old boy named AJ Gonzales who suffers from Fibrodysplasia ossificans progressiva (AKA FOP). This terrible...two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of research labs and hopes that...pathways that impinge upon FOP to deposit their plasmids with Addgene and join us in the fight against ...

  14. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post
    ...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  15. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...are identical for antibodies within certain classification groups called isotypes.  Why does isotype matter...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

  16. What the HEK?

    Type
    Blog Post
    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

  17. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ...the newest plasmid you requested from Addgene...the plasmid that will help you run that last additional...experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick look at your order status on ...of intellectual property (as inventions, ideas, plasmids) among universities and other research institutions...

  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post
    ...This construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins...proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young adult ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

  20. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab...conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them very popular... lab Sign up to receive email alerts when new plasmids from the Boyden lab become available. Visit Ed...
Showing: 421 - 440 of 1038 results