We narrowed to 810 results for: Mal;

...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive...freeze-thaw cycles needed when using the virus. A small aliquot is used to determine functional titer with...aliquot from an amplification done by Addgene.) This small aliquot is not enough to transfect producer cells...

...where one plasmid outcompetes the other, usually a smaller, higher copy plasmid can more effectively titrate...Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead to asymmetrical...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

...blind spots. Blind spots often result in less than optimal user experience and the overall soundness of the...development. Scrum teams plan and break up work into small, well defined chunks, called stories, and work on... to the story being scrapped or broken up into smaller stories. Since this occurs before a single line...

...reagents and process. It is important to note that optimal experimental conditions, including how much plasmid...backbone as Plasmid A—that does not target any mammalian gene. This non-targeting control is critical to...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

... refer to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies...of those CD8+ T cells, require the cells to be minimally altered, so you would not be able to use antibodies... Lastly, stains, based not on antibodies but on small molecule dyes, are useful, most notably when it ...

...pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing is then...ubiquitination activity assays, toxin activity, small molecule binding, growth rate, and many more. See..., Gibson assembly is better suited for creating small, focused scanning libraries. PFunkel: Of prior...

...The new SuperPLDs work with high efficiency in mammalian cells and in vitro, opening up a wide realm of...directed-evolution methods, the activity-based selection in mammalian cells used here could be an attractive strategy...Activity-based directed evolution of a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org...

...Good fixation maintains sample morphology with minimal impact to the target epitope. There are multiple...Sample Preparation This step can involve multiple smaller steps that all help to ensure that antibodies can...Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve sample integrity...

...deliver the best possible videos for free. We’re a small operation, so sometimes it can be a big challenge...premium, so I had to figure out a solution for that. Normally, shots require me to get in close proximity to...good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor stand...

...Approach: Viral RNA is extracted and subject to isothermal amplification by primers that target the E and...RNA is extracted from a sample and subject to isothermal recombinase polymerase amplification (RPA) using...contain reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant temperature...

...science, and can include: equipment, reagents, animals, glassware, and even people. When developing a ...budget bottom line costs may be rather high. That’s normal, because this is where you can start to identify... modeling and collaborations rather than using animals, then that’s a cost that you can eliminate from...

...proteoglycan (HSPG)3 AAV receptor (AAVR)2 Epidermal growth factor receptor (EGFR)3 AAV8 Laminin...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

... will be able to provide letters of reference, informal recommendations, more positive grant application... not know.... 6. Go to science meetings big and small Meetings give you and your research exposure. Use...Eat well- a diet rich in vegetables, foods with minimal processing, and low in empty calories will make...

...Function Antigen presenting cells (APCs) that would normally alert the rest of the immune system to the presence...and IL-10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by...activity of CTLs and NK cells. These cells would normally kill cancer cells but are directed not to through...

...little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence (Drobizhev et...excitation wavelength as it has been shown that even a small incremental change of the excitation wavelength ...Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement...

...choice, and size of the target brain region, the optimal viral titer must be determined empirically (this... weeks over 8 weeks) in order to determine the optimal time period between viral injection and commencement... of neural circuits underlying brain disease in animal models. Nature reviews Neuroscience. 2012;13(4)...

...Differential cytosine editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were ...seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly edited U bases...found that C → G editing was often accompanied by small deletions in their target loci (between 4.0% to ...

...5], Sendai virus [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the...differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors are used to control... While natural transdifferentiation is rare in mammals, an example is observed in the pancreas when excess...

...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information...differing in the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids...

...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...