We narrowed to 588 results for: Ret;
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TypeBlog Post...lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou...
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25 Work From Home Tips for Scientists
TypeBlog Post...defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes ... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...these tools both for pilot experiments and for interpreting the results of studies. When planning AAV experiments... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...“These novel methods will be instrumental in interpreting human variation at large scales and making personalized... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post... mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying a reagent... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer lentivirus... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...force-generating protein machines that can extend and retract over many microns in response to pH changes without... -
Components of CRISPR/Cas9
TypeBlog Post...repeated elements “direct repeats” and the unique stretches of DNA between them “spacers” (see figure below... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...proteins, imaging four colors simultaneously should be pretty straightforward. Thank You to our Guest Blogger... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...dye! Always run a ladder. Ladders allow you to interpret the bands that you get in your sample lanes. Choose... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post..., does not require standard curves, and can theoretically detect a single target molecule in a sample.... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab... -
Antibodies 101: Isotypes
TypeBlog Post... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...