We narrowed to 583 results for: Ret;

1. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ... nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated mRNAs...

2. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...highly optimized bioinformatics pipeline that will return high-quality assembled and circularized plasmids...

3. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer lentivirus...

4. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...transgene is flanked by two ITRs, and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector...

5. Hot Plasmids - January 2023

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   Blog Post

   ...consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl...

6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ...time to network,” Zhang says. “The schedule was pretty busy, but after the meeting, I received a lot of...

7. Scientists Map the SARS-CoV-2-Human Interaction Network

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   Blog Post

   ...lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou...

8. 25 Work From Home Tips for Scientists

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   Blog Post

   ...defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...proteins, imaging four colors simultaneously should be pretty straightforward. Thank You to our Guest Blogger...

10. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ...dye! Always run a ladder. Ladders allow you to interpret the bands that you get in your sample lanes. Choose...

11. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...

12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

13. Hot Plasmids - May 2022

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    Blog Post

    ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

14. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

15. Antibodies 101: Isotypes

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    Blog Post

    ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

16. Antibodies 101: Monoclonal Antibodies

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    ...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

17. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

18. Targeted Mutagenesis with EvolvR

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    Blog Post

    ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

19. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ..., does not require standard curves, and can theoretically detect a single target molecule in a sample....

20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...