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  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

  2. Degrading DNA with Cascade-Cas3

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    Blog Post
    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

  3. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  5. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

  8. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

  9. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  10. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...

  11. An Introduction to Adenovirus

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    Blog Post
    ..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...

  18. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein ...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...
Showing: 421 - 440 of 711 results