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...Your gRNA for CRISPR Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: ...

...Beautiful microscope images which traditionally languished in the pages of thick textbooks are now liberated...

...accessible practical guide which was recently published in Frontiers in Neuroanatomy.  Here are the three...

...surface expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons...

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

... get your plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility in a...

... were lots of fluorescent proteins made from jellyfishes and corals, but all of them fluoresce outside...

...long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These ...

...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....

...antibodies and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional...

..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...

...substitutions don’t fare much better: they largely abolished cleavage by both Cas9 and Cas12 (Kim et al., 2020...

...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...

...Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin...

...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...

...sequence information contributed by the depositor, published backbone sequences, and any known sequences for...

... Just over a month ago I finished up my PhD in Biological and Biomedical Sciences at Harvard University...

...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ...

...Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing...

...backbones that have been designed, tested, and published by academic scientists. To help you find the vector...