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We narrowed to 746 results for: UTY

Showing: 421 - 440 of 746 results

  2. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

  3. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...

  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

  9. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

  14. Pooled Library Amplifications

    Type
    Blog Post
    ...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

  15. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

  18. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...
Showing: 421 - 440 of 746 results