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  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

  4. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

  5. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

  9. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

  11. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

  14. Pooled Library Amplifications

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    Blog Post
    ...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post
    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

  16. Experimenting with New Careers while in Grad School

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    Blog Post
    ...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...

  17. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...

  18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...
Showing: 421 - 440 of 739 results