We narrowed to 752 results for: UTY;

...through programming cells. In the context of therapeutics, if you want to program a cell to identify and... far nothing that has worked. I don’t have the solution. Our paper shows we can easily make orthogonal...

...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...

...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are... in freshwater ponds, provide a good potential solution to this problem. Planarians are easy to buy, cultivate...

...yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine synthesis ...). PubMed PMID: 10436161. 7. Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010)....

...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

...versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells... like the addition of a fluorophore or tag. To utilize HDR, a donor template containing the desired sequence...

...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter. Find ...

...through qualitative data that they are a long-term solution to reproducibility and cost-effectiveness for ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...provides on-site courses in public engagement for institutions and the Royal Society gives training in media...

...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...

...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

...get you down for too long, though, because many solutions have been found to get around this restriction...Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature Protocols, 17...

...enhanced response. Evolution of a new variant Maria and the team set up a directed evolution and screening ...

...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...

... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...

...long-term imaging or advanced techniques like super-resolution microscopy. To make this segment of the color...on the application. Figure 1: Super-resolution imaging of actin filaments with mGold2. Top: ...

...explosion of software tools accompanying the CRISPR revolution. (See omictools for even more.) However, none...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...