We narrowed to 770 results for: VARS

1. Plasmids 101: Gene, enhancer, and promoter trapping

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   ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

2. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

3. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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   ...Do you prefer being a generalist? Working on a variety of projects and in different fields of research...Everyday was the same and I was bored. I wanted more variety and more power to influence the direction of my...

4. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   ...BioGRID ORCS resource will be adaptable to a wide variety of screen formats and contexts and continue to ...improve the accessibility of CRISPR screen data to a variety of users. Many thanks to our guest bloggers from...

5. Management for Scientists: What Makes a Good Manager Anyway?

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   ...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style...

6. Advice for Choosing a Research Project

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   ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...

7. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused...Localization of antibody-combining sites within the variable portions of heavy and light chains. Proceedings...

8. Single-cell tracking of lineage and identity with CellTag

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   ...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ...

9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification...

10. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

11. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... Various Addgenies...

12. Addgene Begins Distribution of Recombinant Antibodies

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    ...the community about how the antibodies work in various applications, enabling you to better plan your ...antibody, the target antigen, and performance in various applications are included in the full webpage. ...

13. RUBY-Red Siliques

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    ...plant cell culture), which is great for a wide variety of applications. But some techniques require you... RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely due ...

14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

15. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ... the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...system could be used to deliver or measure new variables. The sky is really the limit for this emerging...

16. Viral Vectors 101: Transductions

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    ... incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver nucleic...number of ready-to-use viral preps available for a variety of broadly applicable and popular viral vectors...

17. Antibodies 101: Epitope Tags

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    ... for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a variety of antibodies...

18. Don’t Wait To Launch Your Health Science Startup

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    ...management team). Sreyan is a research associate at Harvard Medical School and runs everything science (CSO... get through to a number of experts, including Harvard/MIT faculty, venture capital and angel investors...

19. Advice for Moving Into Sales After Your Science Postdoc

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    ... start somewhere. I knew if I started with the Harvard Business Review or some Chartered Institute of ...further education may allow you to transition into various realms of business.  Thank you to our guest blogger...

20. Extracurricular Activities for a Strong Science Career Path

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    ...resource allows the undecided to learn about a variety of science professions based on their skills and...future regulations impact your work? What design variables need consideration? Enrich your knowledge and ...