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We narrowed to 841 results for: ang

Showing: 421 - 440 of 841 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...phenomenon you will likely want to monitor the voltage change across the membrane of cardiac cells. Similarly...binding a Janelia Fluor (JF). The voltage-dependent changes in rhodopsin modulate the degree of quenching of...  References and Resources   References Beck C, Zhang D, Gong Y. Enhanced genetically encoded voltage ...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed... NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...AAV-containing 40% fraction is collected and buffer exchanged to remove the iodixanol and concentrate the purified...or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the AAV Iodixanol Gradient...correctly. When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...speakers each week, my department and graduate office arranged opportunities to meet with various professionals...volunteering, I was worried about becoming one of those angry, disappointed graduate students because I wasn’t...place to go once a week to get recharged. The fun change of pace helped break up the negativity and allowed...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated by natural...previous gene drive and thereby undo the genetic changes driven by the earlier intervention2. While an initial.... An accidental release would be particularly dangerous for molecular biology and genetics because the...

  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... colonies are an issue. Pros: Very stable, interchangeable with Amp, timesaving for transformations. Con...similar relationship as amp and carb (they are interchangeable). Like carb, spectinomycin is the more stable..." %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley,...

  10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...a characteristic absorption spectra across this range of wavelengths with maximal absorption at 260 nm...present in the sample also absorb light in this range of wavelengths (Figure 1B-D). These other molecules...Proteins absorb light to varying degrees across the UV range used in NanoDrop systems. Peak absorption occurs...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... VSV-G envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, ...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...for later culture: At this point, you will want to hang onto your clones for later use. There are a few ...directionality. Verifying the insert sequence with Sanger Sequencing After identifying a few positive clones...mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing allows you to confirm the...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...quinolone resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic ...diverse, and each phage has a narrow bacterial host range. To further potential clinical applications, Ando...Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials...

  14. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...dressing in costume, and holding competitions (ranging from cook-offs, to corn toss to trivia, and everything...participation numbers for an event you are planning, dangle the carrot (or more popularly- cookies). 3. Do...quiet guy from the lab was such a master drywall hanger?  4. Utilize your office space too Balance point...

  15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit of depositing plasmids to... which is very disappointing. I hope I can help change the situation in the future. Addgene: What advice... they may be able to achieve something that may change the rules of the game. In this regard, I believe...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...physiological processes. Re-engineering GFP to increase its range of color and application Almost as soon as its...domains of a protein that undergoes conformational change. Typically, two fluorescent proteins with overlapping...ion (such as Ca2+) concentrations and pH, using a range of strategies such as FRET, calmodulin, and others...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...virus.  VSV-G is very common due to its broad host range, and increased particle stability; however, the ...

  19. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...that emerged from intensive engagement with a wide range of people at the University of Wisconsin in 2011...chat apps like Slack, Microsoft Teams, and Google Hangouts Chat, resources like Github and Gitlab, and science-specific...reproducibility an ELN should have integrations with a broad range of relevant generic and science-specific tools. ...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...temperature parameters. For example, we found that changing the annealing temperature of the PCR from 60° ...primers can have different annealing efficiencies (Wang et al., 2013). Although you can optimize your primers...: 28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy...
Showing: 421 - 440 of 841 results