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We narrowed to 492 results for: ara-2

Showing: 421 - 440 of 492 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis...multicistronic vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters...at the C-terminus of a 2A element, leading to separation between the end of the 2A sequence and the next...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...to fulfill orders.  The barcoded tubes help to guarantee that no samples get lost during their journey....Addgene Get help with plasmid transformation, preparation, and analysis from our protocols and molecular...

  3. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull. "Codon bias and heterologous...: 346-353. PubMed PMID: 15245907. 5. Maertens, Barbara, et al. "Gene optimization mechanisms: A multi‐...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells." State University...line identity and purity making it easier for comparable studies to be performed on a cell line even decades...preferably some of these vials will be stored in a separate backup liquid nitrogen store to prevent loss from...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...non-specific binding in our IHC condition.   Fig. 2: Schematic of the goldilocks antibody selection process...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens when...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    .... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...the project were able to move forward quickly in parallel. More broadly, we drew on the combined creativity...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger... a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed when these...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2...et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA23.10 (2017): 1592-1599...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional..., with significant contributions from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru.../doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N, Gradinaru V...Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. ...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, ...to one receptor, so it is important to fully characterize the compound, or compounds, you choose to ensure...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... 2008 Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, ...Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: ...

  14. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...from work being done today, is a nearly entirely separate effort from acknowledging its existence. Studying...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional... are technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...

  16. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression...adapted from Farahani et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani PE, et al...for monitoring specific RTK activation in vivo (Farahani et al. 2022). The authors take advantage of the...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and...   Matlashov ME, et al. Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

  17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...also collected data on fluorescent protein characteristics such as the brightness, photostability, maturation...Cranfill et al, 2016; Lambert, 2019). These characteristics are important, but have less priority than ...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...PMC4383620. Botman, Dennis, et al. "In vivo characterisation of fluorescent proteins in budding yeast."...brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry...

  18. Molecular Biology Reference

    Type
    Guide
    ...mrr-hsdRMS-mcrBC) Phi80lacZDeltaM15 Delta-lacX74 recA1 araDelta139 D(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG ...mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA...+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ...mrr-hsdRMS-mcrBC) Phi80lacZM15 Delta-lacX74 recA1 araD139 Delta(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG ...Invitrogen F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ– leu ...indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions...million DNA fragments, which is far too many to separate from each other for the purposes of isolating ...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward... storage buffers used, and other experimental parameters. For example, while freeze thaw is generally ...25073060. 8. Finkelshtein, D., Werman, A., Novick, D., Barak, S., Rubinstein, M. “LDL receptor and its family...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...and virtually never exceeds 40%. While this is comparable or better than the efficiency of most current...ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery method frees...
Showing: 421 - 440 of 492 results