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We narrowed to 591 results for: cel.2;

Showing: 421 - 440 of 591 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... last ~2-7 days at moderate levels. With regard to target cells or organism, some primary cells and differentiated...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids ...secreted into the cellular media, making viral collection fairly easy. Producer cell media can be collected... rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you would like ...just zapping your cells? That really depends on two things. First, what type of cell or organism context...process of transferring foreign nucleic acid into a cell by a viral agent. Transductions are similar to transfections...processes which introduce foreign nucleic acid into cells by any means, but are unique in that they require...

  4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... temporal activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis...about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...PMID: 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free extracts Typically... into a host cell (through a process called transformation), essentially giving the cell all the instructions...to dispose of the live cells. The alternative is to express the protein in a cell-free manner. This is ...biology, especially those at the molecular and cellular level. The reason for this is cost and complexity...electrophoresis to separate DNA or transforming cells with green fluorescent protein, these activities...transcription and translation. Unfortunately, having live cells in a classroom presents equipment needs, such as...

  6. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, D., Hochman...Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable...systems, and often do not fold properly once inside cells.  Developing the HA frankenbody To combat the issues... detects the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody...previously proven to fold and function in living cells. To make these scaffolds specific to different targets...the tagged protein upon probe binding in living cells.  In their proof of concept experiment the Stasevich...mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that the HA frankenbody probe ...

  7. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009. doi: ... there is one problem: how to get that into the cells of interest,” Griesbeck said. Because they are chemical...understand the connection between molecular- and cellular-level events and behavior. It’s a problem that...advantage for observing events taking place inside cells that are on the move, such as T lymphocytes. Griesbeck...-binding protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had...cAMP-responsive element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck...FRET-based sensor proteins, particularly for the intracellular imaging of transition metal ions, such as zinc...

  8. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...negative bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria...complex within the peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates...

  9. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post
    ... and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they would...would continue on with my academic dream of studying cell division. However, after 6 years in the business...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh...

  10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids... Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation...DNA. This bias can be counteracted by growing the cells on solid plates, where each transformant forms its...own colony, uninfluenced by the growth of other cells. For libraries where the plasmids are all very similar...

  15. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide...transfected cells may be considered an entirely new cell line from that of the original parental cells. Positive...cultured mammalian cells. Of course, these mammalian vectors must be compatible with the cell type they are...bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells is fairly...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the...components expressed in trans within the cell for effective replication. Cell lines expressing the Epstein–Barr...mammalian cells To achieve stable transfection, there should be a selective pressure to force cells to incorporate...

  17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post
    ...differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced with the TF pooled...human embryonic stem cells. Transduced stem cells were differentiated and single cell RNA-seq was performed...transduced human embryonic stem cells with the viral TF library, with each cell receiving only 1 TF expression...construct. Cells were cultured following a differentiation protocol before performing single-cell RNA sequencing... 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced cellular states... clusters of individual cells along with color coded nominated reference cell types.  (Adapted from Joung...delivered to each cell, future applications could deliver multiple TFs to each cell, allowing for combinatorial...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...the producer cells and purification protocols often describe a process that begins with cell lysis to release...particles is able to infect more cells (as demonstrated in different cellular environments such as retina ... steps to separate cell culture supernatant containing exo-AVVs from producer cells, and then to enrich...Exosomal vesicles secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic...effectively target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma...Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production...

  19. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been .... This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...yourself greater chances for success.  Build a team of 2 or 3 Don’t go it alone.  You’ll find that no matter...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky...
Showing: 421 - 440 of 591 results