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We narrowed to 803 results for: mak

Showing: 421 - 440 of 803 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In...sequence was inserted or deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you...issue, you may need to select an alternative gRNA. Make sure to check out our validated gRNAs to avoid rolling...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...false positives observed in the cloning process, making it easier to produce the constructs you need. Expression...affecting its translation initiation and instead makes initiation heavily dependent upon the core of a ...to simplify the protein purification process by making it easy for a researcher to quickly generate N-...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...commitment. Another advantage is that this process makes it easy to generate wild type and mutant constructs...content, contains a lot of repeats--any of which could make the PCR step difficult--OR there is no convenient...rather than using a restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a ...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers...normal function. Even low-frequency editing could make a huge difference in DMD, and it’s estimated that...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa...expression (Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although...depends on your experimental system. As we cannot make universal serotype recommendations, the following...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...a given phage will recognize on a host cell. To make matters even more interesting, much of what a phage...display little to no homology to anything else, making it difficult to quickly identify possible gene ...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology.... For editing the genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about...content in the mouse genome and the Cas9 enzyme will make a cut at the target site. This is when it gets exciting...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...characterized by large DNA recognition sites, which makes them highly specific.  Jurassic Park by Michael ...   Star Trek: Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath ...has always served as a vision of the future and to make us reframe our perspective of the current. Although...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...ve been planning, it is a good idea to pause and make sure that your antibody has been appropriately validated...guarantee recognition of the target antigen. You want to make sure that your antibody binds to what you expect... epitope information for some antibodies, which makes it hard to ensure that you have unique antibodies...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...your cells of interest. You should now be able to make smaller adjustments to a set of injections into ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ...our lab so we opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care...can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in...humans and additional improvements are being made to make them even more useful for therapeutic purposes. ...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...characteristics to consider when selecting a dye or ligand to make your selection easier, as well as highlight some... imaging tools in biological research. This does make finding the right dye for your experiment more complicated..., M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox...

  13. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...this diverse group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...that we’ve learned in the lab that will hopefully make your life a little easier. These can range from ...Putting on lab gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and ...

  15. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...get to talk to scientists from around the world making breakthrough discoveries!” I knew right then I ...way that a connected and collaborative community makes not just for a better day at the office but for ...

  16. Deck the Lab 2022!

    Type
    Blog Post
    ...samples from my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...

  18. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...wanted to take a week to celebrate the scientists who make a big impact in open science. Depositors consistently...this year, Addgene depositors quickly stepped up to make COVID-19 reagents available to researchers across...

  19. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...Expressing these chromoproteins in E. coli would make it easy to distinguish colonies from one another...always get a few isolated colonies. I should probably make it a bit more challenging, potentially by using ...

  20. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...sometimes be elusive or have a certain mystique that makes them difficult to approach for the youngster just...people to do science? 16:08 - 22:04 : How have the make-up and scope of your graduate projects changed throughout...
Showing: 421 - 440 of 803 results