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We narrowed to 1,010 results for: RING;

Showing: 221 - 230 of 1010 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...into their scoring functions. Many tools have also opted to mix-and-match different scoring algorithms... by comparing their past results to the designs suggested by each tool. For those who are entering sgRNA...facilitate most experimental applications in CRISPR engineering. When compared to that collective utility, it... and approximate methods. The details of their scoring function can be found in their online tool’s documentation...is by far the most common with most tools also requiring that the user specify an organism. Output diversity... genome”, “large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any ...solution but are often packaged as offline tools requiring expertise in one programming language or another...

  2. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...their biological materials during funding transitions. Enabling resource sharing: Our operating model allows...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene is ...lost amid lab closures or project terminations, ensuring that scientific progress made through years of...structures and indirect cost caps more efficiently. Offering free educational resources: Our eBooks, videos...productivity and efficiency with fewer resources. Fostering new partnerships: We're seeking and expanding ...strengthening our commitment to collaboration and resource sharing, we can navigate this difficult period together...what support would be most valuable from Addgene during this time. Your input will directly shape our response...

  3. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...consideration when titering a lentivirus is the cell line that is used for titering. Typically researchers... my (lentiviral) generation The first step in delivering genes via lentivirus is deciding what packaging... competence can arise from recombination events during viral production and poses a biosafety risk to ... plasmids in order to obtain the best titers. Requiring an additional plasmid for transfection can make... are required to generate RCL. To overcome the titering hurdles of third generation packaging systems,...will choose a relatively permissive cell line for titering in order to obtain the best titer possible. While... One should also consider the specifics of the titering transduction experiment. Media choice, reaction...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... and morphology, rendering their continued use inadvisable for experiments requiring reproducible results...visible and you should always ensure nothing is covering it. Turning on the UV light 3-4 times a week for...remove all jewellery as well as that watch you’re wearing before you start. Also, avoid unnecessary talking...hood. Planning your experiments thoroughly before entering the hood is another great way to prevent contamination...you’ll know exactly what materials you’ll need to bring into the hood and can wipe them all down before ...air flow around your work area. Make sure to only bring required materials into the hood. This will give...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...

  5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...accomplish is to make the engineering of biology much easier. So you could imagine engineering the entirety of ...Stanford develops engineered DNA systems capable of storing data and computing inside living cells. He's also...an organization working to advance biological engineering openly so as to benefit all people and the planet... what we think of as an operating system for engineering an entire cell. Typically when people use plasmids...cell. Until we get a handle on precise, forward engineering of the central dogma at the genome scale, it'...collection of genetic parts for precisely forward-engineering the central dogma across many genes simultaneously...been 40 years since the invention of genetic engineering. What's incredible is the bioeconomy is already...

  6. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... generated will enter the culture medium during subculturing processes or from the air if the cell line...that are generated (while talking or pipetting) during working in the laminar airflow are also among the...source of the problem is the media, used for subculturing, which is available in both liquid and powdered... before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard...separate lab should be made available for cell culturing and maintenance of the cell lines. Providing such...coats. Have separate footwear for the lab to avoid bringing in environmental contaminants.  Figure...Addgenie works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... cell or neuron type may not work well for a neighboring cell type, even within the same brain region ...What AAV vector is best for my specific system?  Sharing data and community concerns AAV vectors are complex...particular importance to these efforts is the open sharing of negative data.  Negative AAV data is highly ...why they don’t share, many express concern about sharing negative data they cannot explain, perhaps out ...therefore actively work to remove the stigma around sharing negative data and instead focus on getting this...if many scientists choose to help each other by sharing their data. So take advantage of it and get your...Lukner, H., Kogermann, K., & Sepp, T. (2021). Data sharing practices and data availability upon request differ...

  8. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... January 2025.  What will the ordering process look like?  The ordering process is split into two steps...   Figure 1: The process of requesting and ordering a Packaged on Request viral vector prep.  ... research and expanded choice when it comes to ordering high-quality materials from the Addgene repository...Retrograde (AAVrg) capsids.  Please be aware that this ordering process will look slightly different from our ...steps, requesting and ordering. This split allows us to provide researchers with increased choice and flexibility...flexibility; while ensuring we can maintain high standards for both our depositors and requestors.  Requesting.... There is no charge for the request process.  Ordering If your request is approved, you will receive ...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...without the undesirable side products generated during chemical synthesis of these molecules. To make ...recombinant protein purification and metabolic engineering. If instead you want to build genetic circuits...biology MoClo applications, but there are more! Engineering disease resistance through gene stacking, increasing...kit has a focus on transformation and genome engineering. This is a specialized system with parts including...Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology...Mitochondria.” BMC Biotechnology, vol. 17, no. 1, Springer Science and Business Media LLC, 13 Nov. 2017. ...Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes.” The Plant Genome, vol. 16,...

  10. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ... she's either day-dreaming about exploring sci-fi worlds or exploring nature in the real world. Additional...ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by ...edits performed over multiple experiments and comparing the robustness, accuracy, and speed with existing... of the sample name. If there was a minor error during processing, the window shows a yellow checked circle...with non-wild type sequence) as determined by comparing the edited trace to the control trace. In the ...Value - When the ICE linear regression is computed during generation of the ICE Score, the Pearson correlation...sizes in the entire edited population of genomes. Hovering over each bar of the indel plot shows the size...
Showing: 221 - 230 of 1010 results