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We narrowed to 928 results for: INA

Showing: 661 - 690 of 928 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was also used...

  4. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...for single and multi-color imaging experiments. Finally, what kind of system will you use to detect your...

  7. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...this quality.   I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...

  9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...will help get your experiments back on track.  One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  10. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...

  14. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience...

  15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  17. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...critical three letters after your name (P-H-D).  Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we...

  19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend...

  22. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS...

  24. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  26. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly...

  28. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded...

  30. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...
Showing: 661 - 690 of 928 results