We narrowed to 928 results for: POR

...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-...guide-independent off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate...

...introduce modifications at the DNA level in a temporally and locally defined manner. This allows scientists...injection Viral vectors allow a tight spatial and temporal control of gene expression manipulation. They ...

...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

...sigh. The problem is that many of these studies report correlations without clearly testing whether a ...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent...

...of AAAS, WCSJ or ECSJ. These meeting are THE opportunity to grow your network. Get to know and get known...or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places...

...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

... Golden Gate assemblies feature an inverse proportionality between the complexity of the assembly (number...The future of assembly DNA assembly methods are important tools for many areas of science, and researchers...

... purposes. We’re here to help with that! Four important factors to consider for any experiment are: ...Efficiency For cloning in particular, another important consideration is: The number of inserts ......

... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

... accidental release. Science relies on popular support, which in turn depends on widespread trust in scientists...environment, they must never be used without popular support2. An accidental release could delay real-world ...

...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...

... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...

...genomes engineering In the Sternberg group’s initial report of VchINT, three separate plasmids and multiple...simple to use, users should keep aware of a few important details. For transposons to be specifically recognized...

...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...assuming a high resource geographic location) Opportunity for real time engagement while allowing for recording...

...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...

...genome. But not all of those 13,000 regions are important for maintaining ESCs. So which of these 13,000...next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population. ...

...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

...contamination. However, this ratio can be misleading. Some reports suggest that the A260/280 ratio does not drop significantly...purification protocol uses phenol, then it is important to confirm that your sample’s absorption peak ...

...plotting the entire data set, plot only the linear portion. This allows you to use a simple linear regression... enough if your unknown lies within the linear portion of the graph. It does restrict the dynamic range...

... days to weeks in transit and some have been transported across oceans and continents. Each deposit may...misread.  2. Track the container - It is just as important to track the location of the container housing...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...