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We narrowed to 922 results for: Por

Showing: 661 - 690 of 922 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  7. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

  8. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  11. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

  12. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  15. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  19. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...plotting the entire data set, plot only the linear portion. This allows you to use a simple linear regression... enough if your unknown lies within the linear portion of the graph. It does restrict the dynamic range...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...

  22. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...assuming a high resource geographic location) Opportunity for real time engagement while allowing for recording...

  23. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...

  25. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  27. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

  28. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a chimeric 5'LTR removes...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The deletion...

  29. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...all of which need to be represented in the same proportion after amplification as they were prior to amplification...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

  30. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...
Showing: 661 - 690 of 922 results