We narrowed to 954 results for: app

1. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different...

2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ...Juan R. Torruella, U.S. First Circuit Court of Appeals. She served as in-house counsel at St. Elizabeth...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ...

3. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...TurboID and miniTurbo podcast segment A fundamental approach to studying membranes, organelles, and other subcellular...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...

6. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given...

7. Why and how to start studying chromatin (with Addgene's help)

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   ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...over or write to us to get them. Even though we’re happy to provide them, that never works on a large scale..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

10. CRISPR 101: Non-Homologous End Joining

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    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error.” The structural...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

12. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...to today, Sample6 has changed focus and names, swapped leadership, and grown to 25 members.  What is...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...

13. Plasmids 101: Yeast Vectors

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    Blog Post

    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

14. Lab Safety for Biosafety Levels One and Two

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    Protocol

    ...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...and uncluttered. Disinfect your workspace with appropriate materials. For example, you may use 70% ethanol...your lab supervisor what is available and the appropriate contact times for each agent. Make sure you have...autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol goes a long way. It ...

15. Addgene: The First Twenty Years

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    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

16. To Each HIS Own

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    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

17. All in a Twist: dsRNA

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    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

18. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

19. What's New in CRISPR - November 2021

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    Blog Post

    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

20. Antibodies 101: Polyclonal Antibodies

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    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...

21. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...It’s at Fred Hutch.” And I hadn’t seen it. So I applied and I got it.” Science writing at Fred Hutchinson...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using...

22. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ... for non-neural cells, ion-based communication happens at a slightly slower scale.  All living cells ...After attending this meeting I had an increased appreciation for the role of ion channels and pumps in developmental...

23. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

24. Plasmid Tools for Microbiome Studies

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    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

25. Summer SciComm Series: Cognitive Load

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    Blog Post

    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...

26. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

27. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the...

28. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...can end with some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In... in images etc)? Did they perform all of the appropriate controls? Refer back to the methods section for...

29. Plasmids 101: Cre-lox

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    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...

30. Plasmids 101: The Wide World of Natural Plasmids

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    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...