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We narrowed to 910 results for: ire;

Showing: 661 - 690 of 910 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from...

  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...conditional knockout mouse, loxP sites are inserted directly into the genome to flank the gene that will be...

  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Therefore, co-infection with a helper adenovirus is required to provide the viral proteins in trans. The Gutless...virus. Purification (2 days) - Purification is required if rAdV is to be used in vivo. The standard method...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...samples; we showed that we can detect the virus directly from infected monkey blood plasma without purification...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

  11. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    .... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  21. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

  24. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

  25. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

  26. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

  27. CRISPR Antimicrobials

    Type
    Blog Post
    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

  29. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

  30. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...
Showing: 661 - 690 of 910 results