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  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...structural complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser...through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which is...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  3. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  7. Typing CRISPR Systems

    Type
    Blog Post
    ...function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ...

  10. Molecular Biology Reference

    Type
    Guide
    ...mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock...Adenine, Cytosine, Guanine and Uracil (U). Uracil replaces thymine in RNA molecules. Every 3 nucleotides ...

  11. Deep Dive: qPCR

    Type
    Blog Post
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

  12. Technical Design of a Western Blot

    Type
    Blog Post
    ...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ... simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose, ...

  16. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR...
Showing: 441 - 460 of 720 results