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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 963 results for: APP

Showing: 441 - 460 of 963 results

  1. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve learned about...and Tol2), each of which has several different applications (mutagenesis screens, transgenic animals, gene...Garcia-Perez J (2010) DNA Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/...Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...cells with no Cas9 should appear green and cells with functional Cas9 should appear less green. Note that ...the population, so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips...by the presence of DNA cleavage products. The appearance of the 428 bp band indicates edits in the BRAF...

  3. Typing CRISPR Systems

    Type
    Blog Post
    ...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...classification system will help you gain a deeper appreciation and understanding of CRISPR, and perhaps provide...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein composition. It is used for a variety of applications such as measuring target protein expression ...stain the intracellular target. When using this approach, avoid fixation-sensitive fluorochromes such as...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...antibodies during the flow cytometry experiment and will appear as a distinct population. After initially gating...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...PAM is so flexible in this state that it doesn’t appear in the crystal structure (Figure 2) at all. When...cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure 3 crystal structure, reflecting the...which stabilizes the last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where...with and better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed...

  8. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...show the interactions between amino acids in an approximate, two-dimensional map. The Baker Lab wanted to...novel, the principles that created it have been applied to numerous other protein design goals, both from...drugs, all developed with their protein design approach. Congratulations to David Baker, Demis Hassabis...

  9. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    ...This edition of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes...piece of advice - get your PI or lab manager’s approval before letting your imagination run loose on the...diorama. Or maybe you want to create a stack of wrapped gifts using life science catalog pages. Make your...

  10. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...of interest (see below for recommend amounts) Appropriate restriction enzyme (see manufacturer's instructions...instructions for proper ammount) Approrpriate restriction digest buffer (see manufacturer's instructions) ...as Addgene's Sequence Analyzer . Determine an appropriate reaction buffer by reading the instructions for...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually 37 °C) for 1 hour. Always...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...

  11. Immunocytochemistry

    Type
    Protocol
    ...PBS, remove the wash, and dispose of it in an appropriate waste container. Fix each well with 500 µL of...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...safety guidelines for disposing of waste in the appropriate container. Wash 3x for 5 min in 500 µL PBS on...permeabilization buffer and dispose of it in an appropriate waste container. Wash 3x for 5 min in 500 µL ... the blocking buffer and dispose of it in an appropriate waste container. Dilute the primary antibody ...the primary antibody and dispose of it in an appropriate waste container. Wash 3x for 5 min in 500 µL ...min at RT in the dark. Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody...

  12. Editor's Choice, July 2016

    Type
    Blog Post
    ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...this post to anyone wondering if and how they can apply FRET in their own research. All posts from July ...Blogger Form and we'll be in contact with you ASAP. Happy Reading! Tyler ...

  13. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...regions in the near future. Additional Resources Apply to Recieve Equipment through Seeding Labs' Instrumental...

  14. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...brains on how they've managed to keep themselves happy during their time in grad school. As you'll learn...student communities 5:59 - 8:16 : What has made you unhappy in grad school? As part of this discussion, we ...to the right that illustrate a grad student's happiness v time throughout graduate school. This is, of...

  15. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...a plasmid or viral origin. The type of cloning approach you choose ultimately will influence the quantity... for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms or ...assembly or Golden gate assembly use a more direct approach, allowing scientists to clone from a smaller amount...

  16. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...Citing This Plasmid" database - and we always appreciate any help we can get. If you know of an article...PMID) for the article that has used this plasmid. Happy researching! If you need any assistance finding ...have made to the repository, email us and we'd be happy to help. Read More Tips from Addgene Plasmids ...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic...feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking for CRISPR...

  19. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better than...good, customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there...

  20. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... make the library even better, we would be very happy to share updated versions of the kit.” Reference...
Showing: 441 - 460 of 963 results