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Showing: 441 - 460 of 706 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...

  3. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...their primary use in the lab. These strains are all based on E. coli K-12 and are considered BSL-1. Table ...potential to recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44...

  6. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger... these tools for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design....

  7. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...reviews on an agent used to promote bone growth based on data voluntarily made available by Medtronic ...organization called Free the Data launched a public database to gather the results of Myriad's genetic tests...

  8. Open Enzyme Collection

    Type
    Collection
    ...harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through...

  9. Viral Production

    Type
    Collection
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ...will drift pretty rapidly upon addition of acid or base. Add only a few drops at a time. Allow them to mix... mg of DNA or 2000 μg. Using the total number of base pairs from all three plasmids, we can determine ...

  15. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... protein tag to the target and using an epitope-based strategy such as Anti-6xHis. You could also consider...L. (2021). Creating clear and informative image-based figures for scientific publications. PLOS Biology...

  16. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...frequency of ‘TTAA’ in the genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for...This allows researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One...

  20. Science as Art

    Type
    Blog Post
    ...Daniel Stoupin is a PhD scientist and photographer based at the University of Queensland, whose outstanding...
Showing: 441 - 460 of 706 results