We narrowed to 712 results for: BASE

1. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

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   Blog Post

   ...Scientific Officer “Choose your PhD lab carefully. Don’t base it solely on the project. Assess whether or not ...

2. How Exercise Purges Stress and Keeps Depression at Bay

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   Blog Post

   ..., and now researchers have reported a mechanism based on studies of transgenic mice that helps to explain...

3. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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   ...organism. We have designed a Golden Gate system based on the daily needs of an advanced molecular plant...

4. Let There Be LITE Plasmids

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   ...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...

5. Harnessing Bacterial Toxins for Allelic Exchange

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   ...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...

6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...development. Stochastic multicolor labeling systems, many based on Brainbow, now exist for a variety of species,...Labeling Drosophila neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow ...

7. Plasmids 101: Restriction Cloning

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   ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...

8. Transferable Skills Guide: Conflict Resolution

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   ...either “personal” or “substantive”. A personality-based conflict occurs when two people cannot agree or ...describes 5 basic approaches to conflict resolution based on various levels of assertiveness and cooperation...

9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   ...sequences for specific cell types can be identified based on the wear-and-tear footprint that they leave on...tools, enhancers were nominated from the genome based on their differential chromatin accessibility across...

10. Plasmids 101: Common Lab E. coli Strains

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    ...their primary use in the lab. These strains are all based on E. coli K-12 and are considered BSL-1. Table ...potential to recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44...

11. Addgene's Top Blog Posts from 2020

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    ... kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger... these tools for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design....

12. Your Top Requested Plasmid in 2016!

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    Blog Post

    ...variety of new tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled...

13. Top 10 Open Science Developments of 2013

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    ...reviews on an agent used to promote bone growth based on data voluntarily made available by Medtronic ...organization called Free the Data launched a public database to gather the results of Myriad's genetic tests...

14. A new platform for fast functional characterization of potentially cancerous mutations

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    ...team assigned the mutations to functional groups, based on how each one impacted cell viability (see figure...data from the MD Anderson Cancer Center patient databases, this research team identified 1,049 perturbations...

15. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

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    ...Design Check Out News Ways to Use CRISPR for Single Base Editing Resources on Addgene.org Browse Our New...

16. Management for Scientists: Seeking Feedback

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    ...Douglas K. Smith or the book on which this article is based. ...

17. AAV Production in HEK293 Cells

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    ...will drift pretty rapidly upon addition of acid or base. Add only a few drops at a time. Allow them to mix... mg of DNA or 2000 μg. Using the total number of base pairs from all three plasmids, we can determine ...

18. Is this the right place for me? 8 tactics for choosing a lab

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    ...scientists make choices based on logic and reason, but often our decisions are based on emotions and assumptions...

19. PiggyBac-ing Through the Genome Editing Field

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    ...frequency of ‘TTAA’ in the genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for...This allows researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One...

20. Antibodies 101: Multiplex Immunofluorescence

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    ... protein tag to the target and using an epitope-based strategy such as Anti-6xHis. You could also consider...L. (2021). Creating clear and informative image-based figures for scientific publications. PLOS Biology...