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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: CAR

Showing: 441 - 460 of 644 results

  5. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...in a 50 mL conical tube and dispose after use. Carefully pour the filtered and diluted cell culture supernatant...Resin will appear compacted after centrifugation. Discard the flow through into a waste container. Add 5 ... 2 min to remove PBS. Repeat Steps 30–31 3x , discarding buffer from the collection tube each time. Place...Centrifuge at 1000 x g for 2 min to collect the sample. Discard column after use. Determine antibody concentration...min at RT . Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Add the...times to mix. Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Repeat...additional times to ensure a full buffer exchange. Discard the flow through into a waste container. Fill the...

  8. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...the routine, I realized that the “9-5” was not as scary as it seemed. The transition into a traditional ...

  18. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ... are some of your favorite proteins purified to carry out your plan. With a little thoughtful planning...
Showing: 441 - 460 of 644 results