..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...immunogenic, a necessity for in vivo studies; (3) is compatible with single gene KO and pooled screening approaches...facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular identifiers...

...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...

.... Nonetheless, Addgene’s annual Deck the Lab competition drew upon the creativity of the community, who...place-winning entry doubled as their lab’s decoration competition, and each lab member used an impressive variety... started last year: The Christmas Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter....bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more...

...Beth Kenkel To complement our RNA Extraction Without A Kit blog post, we've compiled five methods for...methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about ...features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow...

...interviewed fellow Addgenies about their role in the company. One of the things I wanted to do was create content...were a part of the different departments of the company. I asked questions relating to their roles at Addgene...month, I can say with certainty that Addgene is a company that cares - not only in terms of its employees... Addgenies. I could take an active role in the company and not just feel like an additional person each...

...add another layer of complexity to imaging, as they allow one to monitor compartment/protein dynamics or... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...Drosophila, and mammalian cells. Photoconverted GFP is compatible with other FPs since its emission wavelength ...

...expansion, scientists will be able to request an AAV composed of an AAV plasmid in the Addgene repository and...by Addgene for legal approvals and technical compatibility with our production process. This process will...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows:  Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA...

...recombination by KD, B2 and B3 resulted in complete or near-complete excision of the stop sequence. In contrast...toxicity, but with the caveat of partial rather than complete recombination. If you’re looking to use site-specific...this system. Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple...

... the clinic and cures. There is no doubt that companies are swarming to see what can be done with this...the insulin signaling cascades in only 4 months compared to a year or greater with older technologies. ... in the room. He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus...the code of viruses to give information to the computer to fight the known viruses. Bacteria have figured...

...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...donor with 40 bp homology arms. The HDR efficiency compared favorably to using WT Cas9 with the left gRNA ...

...projects spanning from the identification of plant compounds and insecticidal proteins to the development of... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...

...t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and...writer, I was unsure if I should possibly take a composition class in the English Department. He suggested... coming to the writing workshop in D.C. had to compose and submit a manuscript that they were working ...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

...synthetize all compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical...yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: Cells that have...

...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling...PhiC31 irreversibly recombines one of the two competing attP sites to drive expression of either a stop...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...

...some extra guidance to understand the specific components that make up a plasmid and why each is important...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...used in research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid...

... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...size should be a max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...

... to complete a six month work experience called a co-op to receive a degree. Northeastern compiles a list...Thankfully, my managers set aside time for me to complete an online social media certification on social...petting dogs, and winning the office Just Dance competition.    Special thanks to my manager, Sr. Marketing...

...CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured REST... to stably archived large-scale datasets by computational biologists. Figure 1: The BioGRID Open... from humans and other species. By accurately compiling genome-wide datasets, which are often obscured...

...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...deoxyadenosine (A) at the 3' ends of PCR products. The complimentary “T” in the pair comes from a topoisomerase I-linearized...overnight. 4. Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol ...transforming your reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...

...platform that allows us to provide scientists with complete plasmid sequences. After launching our viral service...present SMRT sequencing only works for self-complementary AAVs and the TA-based ligation and tagmentation...molecules in the DNA extract associate with complementary strands, enough double-stranded species exist...adeno-associated virus particles containing complementary polynucleotide chains. J Virol 9(2): 394-396...