We narrowed to 825 results for: EED
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TypeBlog Post...help you. If the organization is large, you will need to be more directed and target someone in a specific...much better chance at an interview –but you still need to nail the job yourself. See the links in the...
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9 Tips to Achieve Success in Academia
TypeBlog Post...interdisciplinary. 3. “There are many different ways to succeed – as loner or gregarious; dictator or laissez-...ways to have a large positive impact in society. We need much, much more science and engineering (STEM) expertise... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post... suggest our content is reaching more people who need it! After a few months, we wanted to share these...of support and independence that allowed me to succeed. As a team, we talked through slide design, discussion... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...into this workflow? CRISPR both speeds up the process and can help weed out a lot of poor drugs before ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence...repetitive region is present in the plasmid, we might need to perform a restriction digest to confirm the size... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Invasiveness Both optogenetic and chemogenetic tools need to be delivered to cells, commonly through viral...Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing their expertise with... -
Fostering communication in remote collaborations
TypeBlog Post...collaboration and immediately discuss the feedback with everyone involved can speed up the future directions of the... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post... easier for you to find and use the plasmids you need. Here’s what you’ll find in this article: CRISPR-based...catalytic activity. As SLs are also known to trigger seed germination of parasitic plants, this approach was... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...master’s degree. I wanted to be a scientist and you need a PhD to be a scientist, or so I thought. So I entered...s-level job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is ... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...the community’s needs, so please let us know what you think. We appreciate your feedback. If you have questions... -
Build Your CRISPR Vocabulary
TypeBlog Post... often used interchangeably with the term sgRNA. Seed sequence: The 8-10 bases on the 3’ end of an RNA...CRISPR Cheat Sheet Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology... -
Year of the Camelid: Antibody Style
TypeBlog Post...cloning and expression from plasmids, due to only needing one open reading frame Ability to access unique...to producing single-domain antibodies without the need for a species that naturally produces heavy-chain... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists... about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post... biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process...experiment. For example, does the fluorescent tag need to be pH stable or withstand a particular solute... -
Choosing a Good Mentor for Scientists
TypeBlog Post...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post..., making AAV starts with some plasmids. You just need three plasmids to start making AAV: the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate, and the transfer plasmid... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures to study these...Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year. Geoff... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...