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We narrowed to 1,034 results for: LAS

Showing: 441 - 460 of 1034 results

  1. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...microbiology class my sophomore year and it was the first time that I was in a lab class where we had ...has been critical to the Broad’s success over the last 11 years and was certainly critical to drawing me...like... when it looks good, that’s a high that can last for several days.” Working with CRISPR Recently...and talk to the people who actually have these plasmids, figure out what they do and what they might be...

  2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...Information Systems Audit and Control Association. Lastly, Carl also serves on the Board of Advisors and ...

  3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...

  4. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services...Service Find Retrograde AAV Browse Optogenetics Plasmids ...

  5. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...tools for molecular and cell biologists over the last two decades, but their use can be limited by a few...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  6. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ... towards cold, we decided to focus on a special class of enzymes expressed by psychrophilic (cold-loving...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment Read our...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine that can...experience experts, our software team tailored Elasticsearch to support the different usage patterns the ...and depositors — each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols... and category. For example, a search scoped to plasmids shows filters for expression and vector type, ...related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions provide...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...their signal strength, and how long their signals last all impact transduction of the message. To study...ERK KTR and red fluorescence localizes to the cytoplasm. When MEK1 and ERK are inactive, the ERK KTR isn...pathway (Raf1, EGRF), the KTR localized to the cytoplasm. This all-optical psMEK1 + ERK KTR-mRuby2 system...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, it could be...GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins in ...

  9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most...typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are visible. These whorls...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-linear dependence...excitation power (Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...several fluorescent proteins of the same spectral class on the microscopy system and in the cell type (or...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

  12. Summer SciComm: Preprints

    Type
    Blog Post
    ...deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in our catalog...Depositing plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside...can include the Addgene ID for their deposited plasmids in the final journal publication or an updated...

  13. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...isotype classes, it is possible to get secondary antibodies generated against isotype subclasses, such ...from a specific species, such as the rabbit IgG class of antibodies. They do so by targeting the Fc region...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

  15. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...sometimes requires intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  16. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ...

  17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

  18. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  20. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... in the laboratory became frustrated with using glass cloning rings to isolate colonies of antibiotic-...growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater surface area (the amount of...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...
Showing: 441 - 460 of 1034 results