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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 815 results for: MAL

Showing: 441 - 460 of 815 results

  1. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...Function Antigen presenting cells (APCs) that would normally alert the rest of the immune system to the presence...and IL-10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by...activity of CTLs and NK cells. These cells would normally kill cancer cells but are directed not to through...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...choice, and size of the target brain region, the optimal viral titer must be determined empirically (this... weeks over 8 weeks) in order to determine the optimal time period between viral injection and commencement... of neural circuits underlying brain disease in animal models. Nature reviews Neuroscience. 2012;13(4)...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Differential cytosine editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were ...seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly edited U bases...found that C → G editing was often accompanied by small deletions in their target loci (between 4.0% to ...

  4. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information...differing in the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids...

  6. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  7. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR ...Miles on everything from AAV prep to targeting to animal models. It was one of our favorite posts from 2022...

  8. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component...

  9. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

  11. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...References 1. Georgieva, Maya V., et al. "Inntags: small self-structured epitopes for innocuous protein tagging... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed...

  12. Editor's Choice, September 2016

    Type
    Blog Post
    ...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has...Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection...

  15. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

  16. Science as Art

    Type
    Blog Post
    ... is has been featured in Time magazine . Other smaller competitions are now taking place and gathering...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...
Showing: 441 - 460 of 815 results