Genome-wide Screening Using CRISPR
Type
Blog Post
...process. While CRISPR is certainly not the first means to carry out so-called “forward genetic screening...
We narrowed to 525 results for: MEA
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Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post... not filter low complexity regions by default, meaning that you do not have to remember to adjust any ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...essentialist links between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...In our experience, good amplification after PCR means successful plasmid assembly (if primer design is... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...CRISPRko results in a complete loss of function meaning that if an essential gene plays a role in the desired... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...RNP method provides scientists with an additional means to deliver CRISPR components to their experimental... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...also too large to be expressed in bacteria, which means their production requires animal sacrifice and precludes... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...? Zeocin is genotoxic when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA... -
An Introduction to Adenovirus
TypeBlog Post...synchronized exposure under these conditions once the measures were lifted (Servellita et al., 2023). This anecdote... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...worthwhile to generalize the idea of “transduction” to mean any transfer of material from the virus to the host... -
MXS Chaining
TypeBlog Post...MXS-chaining also has the advantage of being directional, meaning that (in the previous example) one can control ... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... you know how to do the job. You may think this means you’re guaranteed to get the job, however, many ... -
Lentiviral Vector Uses and Overview
TypeBlog Post...C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb;15(2):78-82... -
Plasmids 101: Colony PCR
TypeBlog Post...because you get the expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...tubes. 1D barcodes are better for tubes that are meant to be read one at a time. 1D barcodes can be purchased... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...outside of our break room. A ring of the chimes means something fun is going on and to get there fast!... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...to help you enter and to have a successful and meaningful career in the grants profession: 1. Build on ... -
Plasmids 101: Protein tags
TypeBlog Post... a commercially available antibody, that doesn’t mean it’s a good one), then your first step towards detecting... -
Bioinformatics at Addgene
TypeBlog Post...experimenting with open source assembly algorithms meant that we already had a clear idea of the necessary...