We narrowed to 810 results for: Mal;

...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component...

...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR ...Miles on everything from AAV prep to targeting to animal models. It was one of our favorite posts from 2022...

...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

...the transition now to being general counsel of a small start-up. For the last 20 years, I was a patent ...very different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. ...

...References 1. Georgieva, Maya V., et al. "Inntags: small self-structured epitopes for innocuous protein tagging... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed...

...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has...Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection...

...opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many...

...there until they mature and changes sex from male to female. Then, it detaches from the gills and latches...

...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

... is has been featured in Time magazine . Other smaller competitions are now taking place and gathering...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old...

... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

...the same in both cases, but activation depends on small molecule activation (chemogenetics) or light (optogenetics.... TL;DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics...

...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

...downloadable in a small file size with a Biorender watermark, which is usually ok for informal settings like...

...it’s a probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years... for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010...

...occurs infrequently and rarely makes it into the small selection of data found in full-length publications...experimental results but, obviously, this is not optimal. What if you could save the figures of interest...

...activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small molecules...

...reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature throughout...Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org ...

.... – CRISPR can be used to generate cell line or animal models which have the precise disease genetics.... drug selectively kills the wildtype line with minimal to no toxicity in the knockout line, it is likely...