We narrowed to 765 results for: Mos
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TypeBlog Post...during tissue collection and homogenization. The most widely known of these products is TRIzol® (also ...researchers can do to combat this issue. First and foremost, be mindful of your extractions- if you need really...
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Transferable Skills Guide: Cross-team Communication
TypeBlog Post...dry lab scientists (or vice versa), then you have almost certainly experienced some of the hurdles I needed...needed to overcome. I eventually pinpointed and (mostly) solved two main problems with Addgene’s cross ... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on...to you (unless they’ve chosen to hide it). While most people use LinkedIn messaging, not everyone likes... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...sharing your science - which can drive away even the most enthusiastic of learners. What contributes to cognitive...audience to take on, just to understand you at the most basic of levels. Concepts This can be obvious, ... -
Antibodies 101: Affinity Reagents
TypeBlog Post... a number of research and clinical applications. Most of the time, when someone speaks about an affinity...thing to go when downsizing an affinity reagent, most of the alternative reagents have reduced immunogenicity... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...was successfully used in conditions that are the most similar to yours. Take a look at the publications... You now know that application validation is the most important factor in antibody selection, but what... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post... is further processed to isolate the antibodies. Most commonly, antibodies of the IgG isotype are used...When to stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...it is unpredictable which fluorescent protein is most photostable for each of these conditions. On top...power used for live-cell imaging. To conclude, the most photostable fluorescent protein can be identified... -
To Each HIS Own
TypeBlog Post...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with... -
Antibodies 101: Conjugation
TypeBlog Post...conjugate class than fluorophores are enzymes. The most common enzyme conjugates are Horseradish Peroxidase...be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...big promise is orthogonality. You can create an almost limitless number of DNA binding proteins with Cas9...genetic parts for complexity. We have characterized almost 600 terminators. That gives us enough hardware ... -
Hot Plasmids - October 2020
TypeBlog Post...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post..., Konermann et al., 2015, Chavez et al., 2015). Most popular methods for CRISPR activation CRISPR activation...activation While SAM, SunTag, and VPR have become the most popular methods for CRISPR activation, research ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied CRISPR system, N. meningitidis...work? Anti-CRISPRs proteins are highly diverse but most block CRISPR activity one of three ways: Inhibit... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute favorite response...Operations Specialist. She said, “I’ve found the most purpose at Addgene being myself. I like being with... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post... the targeting scope of adenine base editors The most commonly used S. pyogenes Cas9 requires the target...ABE7.10 with ABE8.20-m reducing protein expression the most in primary human T cells. While editing single genes... -
Engaging with science and society at pgEd
TypeBlog Post...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...range of clinical manifestations. PMM2-CDG is the most frequent CDG type characterized by deficient phosphomannomutase...mortality. Since glycans play an essential role in almost every aspect of immunology, participating in events...