We narrowed to 568 results for: PAC;

1. Mentoring for Scientists: I Have a Mentor, What Now?

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   Blog Post

   ...Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

2. Zebrafish Plasmid Collection

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   Collection

   ...expression profiling during regeneration. Seurat - R package for analyzing single cell genomics in zebrafish...

3. CRISPR Plasmids - Mammalian Expression

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   Collection

   ...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

4. Plasmids 101: NGS Quality Control for Pooled Libraries

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   Blog Post

   ...commonly uses a custom script derived from the count_spacers.py file from Feng Zhang’s laboratory. There are...

5. Addgene Depositors Get More Citations

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   Blog Post

   ...0.75   125.3 In figure 2, you can see the full impact from using these weighted numbers. Note: this does...

6. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...biology where questions of ethics can have profound impacts on the development of applications with the potential...

7. Pushing the Limits of DNA Assembly

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   Blog Post

   ...placed. With other techniques, when mutations are spaced farther apart, it becomes hard to accurately execute...

8. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ...s important to verify that these changes do not impact translation rate, as the authors of these papers...

9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

   ... systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed PMID: ...

10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ... difficult to use larger enzymes. To reduce the impact of off-target mutations, scientists are using sgRNA...

11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...beyond. These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene. Other...

12. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    Blog Post

    ...type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while...

13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    Blog Post

    ... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

14. 25 Work From Home Tips for Scientists

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    Blog Post

    ... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...

15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ... Targeting three dCas9 molecules to a precisely spaced region allowed sequence replacement rates of approximately...

16. How to Lead a Great Meeting

    Type

    Blog Post

    ... include every person who could potentially be impacted by a project. Instead, invite the experts on the...

17. Advice for Scientists Starting a Lab

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    Blog Post

    ... environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his...

18. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ...Distributing hardware via globally established makerspace networks and training users via workshops, seminars...

19. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...of a nucleus as “linear” is a misnomer. The DNA packed into our chromosomes is very much 3-dimensional...

20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune system,...