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We narrowed to 1,010 results for: PLE

Showing: 441 - 460 of 1010 results

  1. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...built, literally, 'brick-by-brick' to introduce people all around the world to the lives of researchers...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...Unfortunately, unless they’ve worked in a lab, most people don’t get to experience this very human side to... is to help make science mainstream and to show people the very human struggles in the laboratory routine...sharing and laughing at these struggles will bring people together and make these daily challenges a little...that we step on them with bare feet), to connect people with science. And so, “Bricking Science” was born...

  2. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...can gauge if people are happy depositors is if they deposit plasmids more than once. People get hooked ...Flame Awards. What's in a flame?     We’re always pleased when scientists around the world choose to share...Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the... plasmids and have not yet received your award, please contact [email protected] to claim your Blue Flame...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...

  3. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... detection methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject...this resource for the community. There are a few simple steps you can take to input your data for sharing...and assessment of your experimental results. For example, if you visualized expression after AAV injection...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...clear overview of what you need to submit data. Please note that the data hub is currently still in beta...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ..."CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv (2019)... be tagged with different CellTag libraries at multiple timepoints during reprogramming. These library-specific...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...educational materials to our repository of open samples. We are, and have always been, a community resource... to maximize the reach and impact of your work. Please email [email protected] if you would like to ...ideas for new materials to add to the collection, please let us know by filling out this suggestion form... will find useful antibodies for your research. Please consider sharing your experiences with antibodies...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8:e43322. https....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...

  6. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...until you don't, of trying to understand your completely unexpected findings, or understanding why your...thrice-failed experiment, this paper was actually from a completely unexpected finding in a different experiment,...your conflict, you’ll need two other things to complete your story: an introduction, and a resolution.... big-picture problem, by asking your audience a simple question, like “Have you ever thought about how...conflict to get your audience invested in the more complex story, and then build on that in your resolution...journey, stories from patients or other affected people, man/animal vs nature, anthropomorphized animals...

  7. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...say that most people do not recognize its importance or perhaps even know it exists. Couple that with a ...in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each... resources, we seek out opportunities to engage people in science learning. Whether visiting classrooms... 2017 alone, ABRC staff reached more than 4,000 people through our outreach efforts. Our education partnerships...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants...

  8. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am ... mentors -- well, it's time you call upon these people for guidance.  Before even beginning the process...this part might hurt, but if you pitch to enough people, you will find the answer to your viability question...asking to meet. I have cold emailed hundreds of people asking for meetings to discuss miLab.  Although...impressions are everything and you want to leave people remembering your potential as an entrepreneur, ...

  9. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple swapping...dozens of other published FRET-based sensors. For example, his team is now applying it to improve upon an...domains, in practice optimizing the sensors involves plenty of trial and error. Transition metals also present... of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For us, it... developed something useful [enough] that other people start using it. For us, it’s like a test.” References...

  10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...and what it can enable? Boyden: There are many examples where you want to activate two sets of neurons...humanistic and philosophical implications. As an example, a group at Caltech used an optical fiber together... to treat patients? Over a quarter of a million people have some kind of neural implant already. If we...Those are two of the biggest ones. And as a third example, it would be helpful to have molecules with better...three or four hour conversation. The brain is very complex and we've hosted hundreds of visitors in our lab... different from a cell in a dish. I always tell people, 'Be driven by your science.' Looking for Optogenetics...

  11. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...between sessions Like many large conferences with multiple sessions happening at once, virtual conferences...hurdle of getting up and asking in front of many people is lower,” says Bachle. (For presenters, there’...other ways to make your session more engaging, for example, by using short animations and visuals, or asking...make it seem more like you’re connecting with the people,” Abitua says. Poster sessions The poster session...be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables that you...you can join to connect with others. People are already discussing at tables and the event hasn't even...

  12. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... section now lists the multiple PIDs separated by semi-colons. For our example plasmid it will read “lenti-Cas9...require authors to use specific PID formats. For example, several journals like eLife, Nature, and Cell ...these PID formats are new to you, we’ll go through examples to highlight the difference between them using...identifier and RRID formats. Let’s go back to our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down...Bauer (Addgene plasmid # 87155 RRID:Addgene_87155)” Please note that we only provide recommendations for how...

  13. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ... a photosensitizer composed of the protein complex complex and the MG-2I-dL5** fluorogen that can be used...thus forming the full FAP-TAP complex (MG-2I-dL5**).   A FAP-TAP complex will light up if excited with...-TAP MG-2I-dL5** MG-2I-dL5** is a new FAP-TAP complex where dL5** is the FAP. It’s job is to bind MG-...fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite green (MG) dye...

  14. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... requires extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted...time. Both are helping researchers explore the complexity of translational control in cellular physiology...resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat protein binding...constant tracking. You can then directly monitor multiple rounds of translation from this single mRNA; you...cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the coding sequence and...

  15. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example Article Number...However, determining this exactly is difficult. For example, it is very plausible that science published in...research our adjustment controls for them.   So, for example, in 2010, 10 percent of the papers with Addgene...factors that could still be controlled for. As an example, plasmid-related areas of science could be growing...www.neil-t.com/ .   References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...

  16. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...which "profound" seems like an understatement. Multiple speakers from top minds in the stem cell field...just 8 years after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene...the details. The speakers at this meeting shared plenty of details, but they also uniformly stepped back...uncle with Parkinson's, a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family...expensive Dr. Yamanaka explained that it took 50 people nearly 2 years to create the first stem cell line...not a rate-limiting step in stem cell research. People told me they couldn't have done their work without...

  17. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...particular reference sequence if necessary. The example plasmid on the right has a total size of 7.3kb,.... Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...is not just necessary but also an easy process. Please vist Addgene's resource for plasmid verification...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...connections between different brain regions. For example, communication between the hippocampus and the ...dendrites of proximal neuronal cells. In the specific example of episodic memory, a subset of hippocampal neurons...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...that express Cre in only specific cell types, for example only dopaminergic neurons, neuronal tracing has...unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors is the Allen...part of the brain in a less invasive manner . For example, in neuromuscular diseases it would be desirable...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...

  19. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with... management. Invest time in understanding key principles through books, workshops, and online courses ...foster a cohesive, productive environment. For example, in my current role managing a cross-functional...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals... while changing industries and positions. Some people discover they truly enjoy management and choose...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... cell research has prompted the development of multiple virus packaging methods. Across these methods,...transfection step of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging... to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... the components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018...  Multiple Authors...
Showing: 441 - 460 of 1010 results