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...can help with plasmid sharing! Judging by how many prizes were given out during the lunchtime pizza and...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite... that are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy...

...articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...publications alone aren’t enough. Scientists are sharing what they call “raw science” – data sets, code,...researchers to track and share their own libraries and offering another way to consider and quantify the impact...

...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...haven’t already used it yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR...work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase-9 secretion...Addgene and look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale...

...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...you can see in Figure 1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps...hypothetical experiment in the lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured...

...in order to directly show that, for example, the firing of a specific type of neuron in a specific brain...whether you wish to stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the... which results in depolarization and subsequent firing of an action potential. Halorhodopsins (HR) are...may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery method...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

...unmatched customer support, as this is vital to ensuring that our services help accelerate your research...support team that can help you through any search, ordering, technical, or quality control issues. Improving...within the widget, you’ll navigate to articles answering your questions making it easier than ever to get...describe your issue and press send. Doing so will bring your concern directly to our support team’s attention...

..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...and relevance for the efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva... Mary Gearing...

...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...connected to scientists and use the skills they learned during their studies. They suggest that you make time ...Connie Cepko, Tom Ellis, and George Church for answering our burning questions about how they manage and...

...speaker biographies.” During the week of our interview, Hans was also busy preparing materials for an upcoming...took that gamble, and it’s paid off. Work is never boring. “I just keep learning on the job. It keeps it ...mechanism that also looks nice is a great way to bring people in.” Are you interested in careers outside...

...can remain present until cleared by the body. Administering the drug once can result in several hours of... Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to...injection. The main challenge in using ligands is ensuring the agonist is able to cross the blood brain barrier...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

...hydrogen bonds, destabilizing the molecule and favoring the separation of the two helixes. Low pH instead...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple...

...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may...negative selection strategies adopt naturally occurring genes for cloning purposes (antibiotic resistance... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...

...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ... Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...

...stage you could infer that the gene which GFP was monitoring was being expressed in the same pattern. These...result but will still allow for expression pattern monitoring. Enhancer traps Like promoter traps, enhancer...unambiguously identify the gene that they are monitoring as they are directly integrated into it. Enhancer...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture...reducing the read depth necessary when sequencing and lowering the amount of tissue needed per experiment. In...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...

... category already has five blue flame plasmids covering three different epigenetic effectors. The diversity...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...CRISPR History and Development for Genome Engineering  ...  Mary Gearing...

... each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though,...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...

...academic positions. More recently, I have been hearing exit survey data from postdoctoral programs in ... (every 4-6 years is common), but this can also bring new opportunities—projects end and layoffs occur...change entire culture) Specific requirements for hiring more common Likelier to hire on general talent... paths is an important first step. Developing mentoring relationships, especially with peer mentors, to...

...Vandenberghe. We’re excited to grow the team and bring on scientists with a diverse set of experiences....scientist that helps with different aspects of manufacturing and quality, especially with regard to compliance...journey in biochemistry made the foray into genetics during graduate school at MIT. “I read some of the seminal...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...