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We narrowed to 1,006 results for: RING

Showing: 441 - 460 of 1006 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...bound repressor then prevents transcription from occurring, which means that transcription is now OFF.   ... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture... al. Using Binary Systems Together You may be wondering, “why is it necessary to have all 3 of the different...experiments! This post was originally written by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022...Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS...

  2. Summer Fun at Addgene!

    Type
    Blog Post
    ...Series. We’ll be covering the basics of science communication, tips and tricks for sharing your science with...Addgene TikTok at the end of this month! We’ll be sharing our educational resources and tips and tricks for... now! (Bonus: Tim kindly offered to finishing answering all submitted questions via blog post, which we...

  3. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why...the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had on the podcast... of medical school. 7:05 - 10:55 : Alex's focus during graduate school - DNA damage and longevity. 10:...

  4. Podcast: Interview with Addgene's Founders

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    Blog Post
    ...successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing Addgene’s ...Future, the research team, and the importance of sharing information/knowledge Outro Many thanks to our...received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master of Arts ...Bioworks. He also holds a SB and a Masters in Engineering from MIT, both in Computer Science.   Special...

  5. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...I can bring to Addgene -- that comfort with saying, “if we do this, then what?” I hope to bring that business...my personality. Another big part of my role is bringing experience and judgement to a wide variety of ...business world from the mindset of a lawyer can bring a new perspective to an organization. I'm used to...

  6. Addgene’s Top 10 Blog Posts from 2014

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    Blog Post
    ... grown steadily during 2014. We were excited to have more and more scientists offering to share their ...Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic...Margo R. Monroe Best wishes for your research during this holiday season and we look forward to seeing...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post
    ...are thought to interrogate dsDNA for proper base pairing through minor grove contacts, and thus do not read... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated...upstream probes and four down-stream probes, each differing only in the base at the ligation junction. Thus...prepared, each with a different complement strand covering all 16 possible NN pairs in the template. The ...The probes were designed such that each possible pairing resulted in a product of a different length, with...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...protein can also influence antibody binding by altering the relative concentration of potential antibody...to validate that your antibody is specifically capturing your RNA-binding protein of interest. So you ...antibody’s trustworthiness. You may also consider sharing your validation attempts by other means, such as.... These outlets can be particularly useful for sharing negative data. Negative data often goes overlooked...antibody (perhaps one from Addgene!), consider sharing your results so that others in the community can...

  9. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...on how they've managed to keep themselves happy during their time in grad school. As you'll learn, grad... hard about picking a good mentor, and begin considering career options early. Tune in for great advice... in grad school? As part of this discussion, we bring up a performance Ben and Niroshi organized for Tyler's...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...

  10. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...can help with plasmid sharing! Judging by how many prizes were given out during the lunchtime pizza and...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite... that are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy...

  11. Make a Splash: Notions of Scientific Impact Are Evolving

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    Blog Post
    ...articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...publications alone aren’t enough. Scientists are sharing what they call “raw science” – data sets, code,...researchers to track and share their own libraries and offering another way to consider and quantify the impact...

  12. Your Top Requested Plasmid in 2015!

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    Blog Post
    ...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...haven’t already used it yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR...work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase-9 secretion...Addgene and look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale...

  13. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...you can see in Figure 1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps...hypothetical experiment in the lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured...

  15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...in order to directly show that, for example, the firing of a specific type of neuron in a specific brain...whether you wish to stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the... which results in depolarization and subsequent firing of an action potential. Halorhodopsins (HR) are...may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery method...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  16. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter the table...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

  17. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...unmatched customer support, as this is vital to ensuring that our services help accelerate your research...support team that can help you through any search, ordering, technical, or quality control issues. Improving...within the widget, you’ll navigate to articles answering your questions making it easier than ever to get...describe your issue and press send. Doing so will bring your concern directly to our support team’s attention...

  18. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...and relevance for the efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva... Mary Gearing...

  19. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...connected to scientists and use the skills they learned during their studies. They suggest that you make time ...Connie Cepko, Tom Ellis, and George Church for answering our burning questions about how they manage and...

  20. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ... Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...
Showing: 441 - 460 of 1006 results