We narrowed to 583 results for: Ret;

1. GCE4All: Making Genetic Code Expansion Accessible

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   ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

2. Plasmids 101: Screens vs. Selections

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   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

5. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

6. RaPID Detection of RNA-protein Interactions

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   ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

7. RANbodies: Reporter Nanobody Fusions

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   ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

8. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

9. Visualizing Protein Turnover In Situ

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   ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...

10. Developing Lab Management Software for Biology

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    ...plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the...

11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ...storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA library, but this should...

12. Antibodies 101: Selecting the Right Antibody

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    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

13. Transferable Skills Guide: Problem Solving

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    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...

14. Antibodies 101: Polyclonal Antibodies

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    ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

15. Plasmid Tools for Microbiome Studies

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    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

17. Human Germline Editing Using CRISPR

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    ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

18. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

20. CRISPRainbow and Genome Visualization

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    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...