We narrowed to 588 results for: Ret;

1. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

2. Plasmids 101: Screens vs. Selections

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   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

3. Hot Plasmids - May 2022

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   ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

4. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

5. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

6. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

7. Human Germline Editing Using CRISPR

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   ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

8. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the...

9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...

10. CRISPRainbow and Genome Visualization

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    ...Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting capacity. By using ...

11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...mutants coding for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995...

12. RaPID Detection of RNA-protein Interactions

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    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

13. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

14. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

15. Visualizing Protein Turnover In Situ

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    ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...

16. To Each HIS Own

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    ... specific proteins within a system. This can be pretty complicated given that a single cell has tens of...

17. Plasmid Tools for Microbiome Studies

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    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

19. Antibodies 101: Selecting the Right Antibody

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    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

20. Transferable Skills Guide: Problem Solving

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    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...