We narrowed to 728 results for: STO;
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TypeBlog Post...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...
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Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to ... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...However, ART is not an HIV-1 cure. In patients who stop ART, viral levels will soon skyrocket back to pre-treatment...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription... -
Hot Plasmids: Spring 2025
TypeBlog Post... to the new genomic locus. Image courtesy of Christopher Fell, PhD. After minimizing the system's...enhancer-AAV constructs are now available as in-stock viral preps using the systemic PHP.eB capsid, to... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1 ✓ ✓ AAV2... Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies...platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Fire AZ (2014) Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis... GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic Modifications inCaenorhabditis elegansvia... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity... which would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory...Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer... -
New Antibodies in the Lab Animation!
TypeBlog Post...ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog Post...grade DNA! This is just the first of many milestones Addgene is poised to reach in the near future... -
2022 Depositor Week
TypeBlog Post... of Fame Thursday: 8 Great Depositor Research Stories Friday: Specialized Depositor Shout-Out We'll... -
It’s CRISPR Week at Addgene!
TypeBlog Post... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using... -
Addgene's 20th Anniversary Party
TypeBlog Post...without recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty ... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...tiny creatures do tend to come with a rather astonishing ability to...well, annoy. Fly-related Issues...