We narrowed to 609 results for: She

1. What Do I Do Now? Academic v. Non-Academic Career Decisions

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   ..., interests and passions is another great way to shed light on what paths to pursue. Spend some time really...

2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a...

3. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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   ...which is equally important to report. We recently published a paper in Frontiers in Neuroanatomy and summarized...

4. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ...well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand, especially...

5. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

6. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   ...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...

7. Plasmids 101: Origin of Replication

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   ...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...

8. Antibodies 101: Chimeric Antibodies

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   ...Protein B. The antibodies are both extensively published, knockout validated, and work in your application...

9. What the HEK?

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   ...it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney and is...

10. Addgene Begins Distribution of Recombinant Antibodies

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    ...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...

11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided...

12. Plasmid Cloning by PCR

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    ...check out that post if you need a more detailed refresher on the downstream steps. For this example, we...

13. Another Pathway into Cells: iTOP

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    ...protein purification (once a protocol has been established) may be less laborious than viral purification...

14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    ...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...

15. The Power Behind NGS Plasmid Validation: seqWell

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    ...experiment, the DNA sample being sequenced must be sheared into smaller fragments that are then barcoded for...

16. Creating accessible biology activities in schools with BioBits

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    ...freeze-dried into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production...

17. The Importance of High Titer for AAV Transductions

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    ...definitely also matters). Check out this post for a refresher on AAV titers Making contact with a cell Although...

18. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...properties. Find these voltage biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ...

19. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...Bulina, M. E., Chudakov, D. M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V.,...

20. Visualizing Translation at the Single Molecule Level

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    ...causing it to appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red...