Antibodies 101: Chimeric Antibodies
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Blog Post
...Protein B. The antibodies are both extensively published, knockout validated, and work in your application...
We narrowed to 609 results for: She
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What the HEK?
TypeBlog Post...it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney and is... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...which is equally important to report. We recently published a paper in Frontiers in Neuroanatomy and summarized... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS... -
Plasmids 101: Origin of Replication
TypeBlog Post...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand, especially... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post..., interests and passions is another great way to shed light on what paths to pursue. Spend some time really... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided... -
Plasmid Cloning by PCR
TypeBlog Post...check out that post if you need a more detailed refresher on the downstream steps. For this example, we... -
Another Pathway into Cells: iTOP
TypeBlog Post...protein purification (once a protocol has been established) may be less laborious than viral purification... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...properties. Find these voltage biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...stain within their membrane, while the stain is washed from the gram negative bacteria’s thinner membrane... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...freeze-dried into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production... -
The MTA at Addgene
TypeBlog Post...One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which your material will be distributed...