Plasmids 101: Protein tags
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Blog Post
... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.
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We narrowed to 508 results for: Trac
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Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most... -
Plasmids 101: Codon usage bias
TypeBlog Post...properly. For example Pechmann and Frydman found that tracts of non-optimal codons are associated with specific... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ... -
How to Write a Scientific Review Article
TypeBlog Post...This is normal and it means you’re on the right track! Get feedback After you’re done editing, send it... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people to do ... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent... -
Bioinformatics at Addgene
TypeBlog Post...data for our DNA-based reagents. Not only do we extract as much information as possible from depositing... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...strengths. . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...Confirmation Pick 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by... -
QC Sequencing Technologies at Addgene
TypeBlog Post...the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...Glossary Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name ... -
Hot Plasmids: Fall 2024
TypeBlog Post...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...in vivo transduction of AAV serotypes generally tracks between strains of mice. This suggests that a small...