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We narrowed to 762 results for: UTY

Showing: 441 - 460 of 762 results
  1. Pooled Library Amplifications

    Type
    Blog Post
    ...500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency (Figure ...Figure 2).    Figure 2: Example of a serial dilution to estimate transformation efficiency. Figure used...
  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...improve data quality, as it allows for better resolution of positive and negative populations, Careful...
  3. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...long-term imaging or advanced techniques like super-resolution microscopy. To make this segment of the color...on the application. Figure 1: Super-resolution imaging of actin filaments with mGold2. Top: ...
  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...the intramolecular dynamics of the proteins in solution and when associated with microtubules. ...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...
  5. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and...experience. Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and...
  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...
  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post
    ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...
  8. AAVs in Retinal Gene Therapy

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    Blog Post
    ...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...
  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...
  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...
  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...molecules. Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), ...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...
  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced...method to dissect the individual and shared contributions of transcript isoforms to neuronal physiology...
  13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...
Showing: 441 - 460 of 762 results