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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 749 results for: UTY

Showing: 441 - 460 of 749 results

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...mutually exclusive elements. Labs have successfully utilized 2A and IRES elements within the same multicistronic...developing the clever experiments and innovative solutions necessary for advancing the field. References...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...hopefully only the beginning of a gene therapy revolution and the development of personalized medicine..... AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced...method to dissect the individual and shared contributions of transcript isoforms to neuronal physiology...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, ...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...molecules. Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), ...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...

  16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...accurate than short-read sequencing. At Addgene, we routinely use Nanopore technology to sequence our packaged...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

  19. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK systems is that...bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...
Showing: 441 - 460 of 749 results